Simultaneous bioremediation and biodetection of mercury ion through surface display of carboxylesterase E2 from Pseudomonas aeruginosa PA1

doi:10.1016/j.watres.2016.07.053

	Simultaneous bioremediation and biodetection of mercury ion through surface display of carboxylesterase E2 from Pseudomonas aeruginosa PA1
	Yin, Kun 1,3; Lv, Min1 ; Wang, Qiaoning 1; Wu, Yixuan 1; Liao, Chunyang2 ; Zhang, Weiwei 1; Chen, Lingxin1
发表期刊	WATER RESEARCH
ISSN	0043-1354
	2016-10-15
卷号	103 页码:383-390
关键词	Carboxylesterase E2 Surface Display Mercury Bioremediation Biodetection
DOI	10.1016/j.watres.2016.07.053
英文摘要	Mercury is a toxic heavy metal and presents significant threats to organisms and natural ecosystems. Recently, the mercury remediation as well as its detection by environmental-friendly biotechnology has received increasing attention. In this study, carboxylesterase E2 from mercury-resistant strain Pseudomonas aeruginosa PA1 has been successfully displayed on the outer membrane of Escherichia coli Top10 bacteria to simultaneously adsorb and detect mercury ion (Hg2+). The transmission electron microscopy analysis shows that Hg2+ can be absorbed by carboxylesterase E2 and accumulated on the outer membrane of surface-displayed E. coli bacteria. The adsorption of Hg2+ followed a physicochemical, equilibrated and saturatable mechanism, which well fits the traditional Langmuir adsorption model. The surface-displayed system can be regenerated through regulating pH values. As its activity can be inhibited by Hg2+, carboxylesterase E2 has been used to detect the concentration of Hg2+ in water samples. The developed surface display system will be of great potential in the simultaneous bioremediation and biodetection of environmental mercury pollution. (C) 2016 Elsevier Ltd. All rights reserved.
文章类型	Article
资助机构	National Natural Science Foundation of China(21575159 ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA11020702) ; Thousand Young Talents Program of China ; 21522706 ; 21275158)
收录类别	SCI
语种	英语
关键词[WOS]	HEAVY-METAL IONS ; CELL-SURFACE ; POTENTIAL APPLICATION ; ENZYME BIOSENSOR ; BIOSORPTION ; ADSORPTION ; POLLUTION ; REMOVAL ; DNA ; INHIBITION
研究领域[WOS]	Engineering ; Environmental Sciences & Ecology ; Water Resources
WOS记录号	WOS:000383292100041
引用统计	被引频次：93[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/17210
专题	中国科学院海岸带环境过程与生态修复重点实验室中国科学院海岸带环境过程与生态修复重点实验室_污染过程与控制实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Yin, Kun,Lv, Min,Wang, Qiaoning,et al. Simultaneous bioremediation and biodetection of mercury ion through surface display of carboxylesterase E2 from Pseudomonas aeruginosa PA1[J]. WATER RESEARCH,2016,103:383-390.
APA	Yin, Kun.,Lv, Min.,Wang, Qiaoning.,Wu, Yixuan.,Liao, Chunyang.,...&Chen, Lingxin.(2016).Simultaneous bioremediation and biodetection of mercury ion through surface display of carboxylesterase E2 from Pseudomonas aeruginosa PA1.WATER RESEARCH,103,383-390.
MLA	Yin, Kun,et al."Simultaneous bioremediation and biodetection of mercury ion through surface display of carboxylesterase E2 from Pseudomonas aeruginosa PA1".WATER RESEARCH 103(2016):383-390.