| Differentiation-inducing and anti-proliferative activities of isoliquiritigenin and all-trans-retinoic acid on B16F0 melanoma cells: Mechanisms profiling by RNA-seq | |
| Chen, XY; Yang, M; Hao, WJ; Han, JC; Ma, J; Wang, CX; Sun, SG; Zheng, QS; Zheng, QS (reprint author), Binzhou Med Univ, Yantai 264003, Shandong, Peoples R China.; Sun, SG (reprint author), Northwest A&F Univ, Yangling 712100, Peoples R China. Email:sunsg@nwsuaf.edu.cn; zqsyt@sohu.com | |
| 发表期刊 | GENE
 |
| ISSN | 0378-1119 |
| 2016-10-30 | |
| 卷号 | 592期号:1页码:86-98 |
| 关键词 | Isoliquiritigenin All-trans-retinoic Acid Melanoma Differentiation Rna-seq Ros |
| DOI | 10.1016/j.gene.2016.07.052 |
| 产权排序 | [Chen, Xiaoyu; Sun, Shiguo] Northwest A&F Univ, Coll Sci, Yangling 712100, Shaanxi, Peoples R China; [Yang, Ming] BGI Shenzhen, BGI Tech, Shenzhen 518083, Guangdong, Peoples R China; [Hao, Wenjin; Han, Jichun; Ma, Jun; Zheng, Qiusheng] Binzhou Med Univ, Yantai 264003, Shandong, Peoples R China; [Wang, Caixia] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai 264003, Peoples R China |
| 作者部门 | 海岸带生物学与生物资源利用所重点实验室 |
| 英文摘要 | Melanoma is a cancer that arises from melanocytes, specialized pigmented cells that are found predominantly in the skin. The incidence of malignant melanoma has significantly increased over the last decade. With the development of therapy, the survival rate of some kind of cancer has been improved greatly. But the treatment of melanoma remains unsatisfactory. Much of melanoma's resistance to traditional chemotherapy is believed to arise intrinsically, by virtue of potent growth and cell survival-promoting genetic alteration. Therefore, significant attention has recently been focused on differentiation, therapy, as well as differentiation inducer compounds. In previous study, we found isoliquiritigenin (ISL), a natural product extracted from licorice, could induce B16F0 melanoma cell differentiation. Here we investigated the transcriptional response of melanoma differentiation process induced by ISL and all-trans-retinoic acid (RA). Results showed that 390 genes involves in 201 biochemical pathways were differentially expressed in ISL treatment and 304 genes in 193 pathways in RA treatment. Differential expressed genes (DGEs, fold-change (FC) >= 10) with the function of anti-proliferative and differentiation inducing indicated a loss of grade malignancy characteristic. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated glutathione metabolism, glycolysis/gluconeogenesis and pentose phosphate pathway were the top three relative pathway perturbed by ISL, and mitogen-activated protein kinase (MAPK) signaling pathway was the most important pathway in RA treatment. In the analysis of hierarchical clustering of DEGs, we discovered 72 DEGs involved in the process of drug action. We thought Cited1, Tgm2, Xaf1, Cd59a, Fbxo2, Adh7 may have critical role in the differentiation of melanoma. The evidence displayed herein confirms the critical role of reactive oxygen species (ROS) in melanoma pathobiology and provides evidence for future targets in the development of next-generation biomarkers and therapeutics. (C) 2016 Elsevier B.V. All rights reserved. |
| 文章类型 | Article |
| 资助机构 | National Natural Science Foundation of China(31471338) ; Binzhou Medical University(BY2014KYQD01) ; Xinjiang Production and Construction Corps Funds(2015BD005) |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | CUTANEOUS MALIGNANT-MELANOMA ; STEM-CELLS ; NEOPLASTIC TRANSFORMATION ; PHENOTYPIC ALTERATIONS ; TRANSCRIPTION FACTOR ; GROWTH-INHIBITION ; GATA3 EXPRESSION ; NADPH OXIDASE ; BREAST-CANCER ; IN-VITRO |
| 研究领域[WOS] | Genetics & Heredity |
| WOS记录号 | WOS:000383304600012 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/17192 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 通讯作者 | Zheng, QS (reprint author), Binzhou Med Univ, Yantai 264003, Shandong, Peoples R China.; Sun, SG (reprint author), Northwest A&F Univ, Yangling 712100, Peoples R China. Email:sunsg@nwsuaf.edu.cn; zqsyt@sohu.com |
| 推荐引用方式 GB/T 7714 | Chen, XY,Yang, M,Hao, WJ,et al. Differentiation-inducing and anti-proliferative activities of isoliquiritigenin and all-trans-retinoic acid on B16F0 melanoma cells: Mechanisms profiling by RNA-seq[J]. GENE,2016,592(1):86-98. |
| APA | Chen, XY.,Yang, M.,Hao, WJ.,Han, JC.,Ma, J.,...&zqsyt@sohu.com.(2016).Differentiation-inducing and anti-proliferative activities of isoliquiritigenin and all-trans-retinoic acid on B16F0 melanoma cells: Mechanisms profiling by RNA-seq.GENE,592(1),86-98. |
| MLA | Chen, XY,et al."Differentiation-inducing and anti-proliferative activities of isoliquiritigenin and all-trans-retinoic acid on B16F0 melanoma cells: Mechanisms profiling by RNA-seq".GENE 592.1(2016):86-98. |
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| Differentiation-indu（1419KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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