| Inhibition of biofilm in Bacillus amyloliquefaciens Q-426 by diketopiperazines | |
Wang, JH ; Yang, CY; Fang, ST; Lu, J; Quan, CS; Wang, JH (reprint author), Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China.; Wang, JH (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao, Peoples R China.; Quan, CS (reprint author), Dalian Nationalities Univ, Life Sci Coll, Dalian 116600, Peoples R China. Email:jhwang@yic.ac.cn; mikyeken@dlnu.edu.cn
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| 发表期刊 | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
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| ISSN | 0959-3993 |
| 2016-09-01 | |
| 卷号 | 32期号:9 |
| 关键词 | Biofilm Diketopiperazines (Dkps) Extracellular Polymeric Substance (Eps) Real-time Pcr Bacillus Amyloliquefaciens Q-426 |
| DOI | 10.1007/s11274-016-2106-4 |
| 产权排序 | [Wang, Jian-Hua; Lu, Jian] Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Yang, Cui-Yun; Fang, Sheng-Tao] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China; [Wang, Jian-Hua] Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao, Peoples R China; [Quan, Chun-Shan] Dalian Nationalities Univ, Life Sci Coll, Dalian 116600, Peoples R China |
| 作者部门 | 海岸带生物学与生物资源利用所重点实验室 |
| 英文摘要 | Biofilm formation can make significant effects on bacteria habits and biological functions. In this study, diketopiperazines (DKPs) produced by strain of Bacillus amyloliquefaciens Q-426 was found to inhibit biofilm formed in the gas-liquid interface. Four kinds of DKPs were extracted from B. amyloliquefaciens Q-426, and we found that 0.04 mg ml(-1) DKPs could obviously inhibit the biofilm formation of the strain. DKPs produced by B. amyloliquefaciens Q-426 made a reduction on extracellular polymeric substance (EPS) components, polysaccharides, proteins, DNAs, etc. Real-time PCR was performed to determine that whether DKPs could make an obvious effect on the expression level for genes related to biofilm formation in the strain. The relative expression level of genes tasA, epsH, epsG and remB which related to proteins, extracellular matrix, and polysaccharides, were downregulated with 0.04 mg ml(-1) DKPs, while the expression level of nuclease gene nuc was significantly upregulated. The quantitative results of the mRNA expression level for these genes concerted with the quantitative results on EPS levels. All of the experimental results ultimately indicated that DKPs could inhibit the biofilm formation of the strain B. amyloliquefaciens Q-426. |
| 文章类型 | Article |
| 资助机构 | Key Laboratory of Marine Environmental Corrosion and Bio-fouling(MCKF201402) ; Institute of Oceanology, Chinese Academy of Sciences ; One Hundred-Talent Plan of Chinese Academy of Sciences (CAS) ; Research Program of CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation(1189010002) |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | PSEUDOMONAS-AERUGINOSA BIOFILMS ; CYCLIC DIPEPTIDES ; MICROBIAL BIOFILMS ; FUSARIUM-OXYSPORUM ; BACTERIAL BIOFILM ; EXTRACELLULAR DNA ; MARINE FUNGUS ; CROSS-TALK ; ANTIBACTERIAL ; COMPOUND |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| WOS记录号 | WOS:000382148200004 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/17191 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 通讯作者 | Wang, JH (reprint author), Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China.; Wang, JH (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao, Peoples R China.; Quan, CS (reprint author), Dalian Nationalities Univ, Life Sci Coll, Dalian 116600, Peoples R China. Email:jhwang@yic.ac.cn; mikyeken@dlnu.edu.cn |
| 推荐引用方式 GB/T 7714 | Wang, JH,Yang, CY,Fang, ST,et al. Inhibition of biofilm in Bacillus amyloliquefaciens Q-426 by diketopiperazines[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2016,32(9). |
| APA | Wang, JH.,Yang, CY.,Fang, ST.,Lu, J.,Quan, CS.,...&mikyeken@dlnu.edu.cn.(2016).Inhibition of biofilm in Bacillus amyloliquefaciens Q-426 by diketopiperazines.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,32(9). |
| MLA | Wang, JH,et al."Inhibition of biofilm in Bacillus amyloliquefaciens Q-426 by diketopiperazines".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 32.9(2016). |
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