Institutional Repository of Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences (KLCEP)
| A sensitive fluorescent biosensor for the detection of copper ion inspired by biological recognition element pyoverdine | |
Yin, K; Wu, YX; Wang, SS; Chen, LX ; Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China. lxchen@yic.ac.cn
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| 发表期刊 | SENSORS AND ACTUATORS B-CHEMICAL
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| ISSN | 0925-4005 |
| 2016-09-01 | |
| 卷号 | 232页码:257-263 |
| 关键词 | Copper Ion Fluorescent Biosensor Pyoverdine Electron Transfer |
| DOI | 10.1016/j.snb.2016.03.128 |
| 产权排序 | [Yin, Kun; Wu, Yixuan; Wang, Shasha; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat; [Yin, Kun; Wu, Yixuan; Wang, Shasha] Univ Chinese Acad Sci |
| 作者部门 | 中科院海岸带环境过程与生态修复重点实验室 |
| 英文摘要 | The environmental copper pollution seriously threatens the health of organisms and the safety of ecosystem. Therefore, the development of a simple and sensitive method to detect copper ion is very important. In this study, we have developed a fluorescent biosensor based on biological recognition element pyoverdine to selectively detect copper ion. The fluorescence of pyoverdine is quenched obviously after binding with copper ion. A good linearity within the range of 0.2-10 mu M (R= 0.997) is attained and the detection limit is 50 nM. The biosensor has been successfully utilized for the detection of copper ion in drinking water, seawater and bio-samples and the results agree well with those obtained by the inductively coupled plasma mass spectrometry. Therefore, the established biosensor is a creditable method to detect copper ion with high sensitivity and selectivity, which can be utilized as a powerful tool to monitor copper pollution in the environment. (C) 2016 Elsevier B.V. All rights reserved. |
| 文章类型 | Article |
| 资助机构 | National Natural Science Foundation of China(21575159 ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA11020702) ; 21275158) |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | SOL-GEL GLASS ; PSEUDOMONAS-AERUGINOSA ; COLORIMETRIC DETECTION ; SELECTIVE DETECTION ; LIVING CELLS ; SENSOR ; CU2+ ; SIDEROPHORE ; CHEMOSENSOR ; DISEASE |
| 研究领域[WOS] | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
| WOS记录号 | WOS:000375483000032 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/17075 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室 |
| 通讯作者 | Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China. lxchen@yic.ac.cn |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat 2.Univ Chinese Acad Sci |
| 推荐引用方式 GB/T 7714 | Yin, K,Wu, YX,Wang, SS,et al. A sensitive fluorescent biosensor for the detection of copper ion inspired by biological recognition element pyoverdine[J]. SENSORS AND ACTUATORS B-CHEMICAL,2016,232:257-263. |
| APA | Yin, K,Wu, YX,Wang, SS,Chen, LX,&Chen, LX .(2016).A sensitive fluorescent biosensor for the detection of copper ion inspired by biological recognition element pyoverdine.SENSORS AND ACTUATORS B-CHEMICAL,232,257-263. |
| MLA | Yin, K,et al."A sensitive fluorescent biosensor for the detection of copper ion inspired by biological recognition element pyoverdine".SENSORS AND ACTUATORS B-CHEMICAL 232(2016):257-263. |
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