Institutional Repository of Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences (KLCEP)
| Near-Infrared Fluorescence Probe for in Situ Detection of Superoxide Anion and Hydrogen Polysulfides in Mitochondrial Oxidative Stress | |
Huang, Y; Yu, FB; Wang, JC; Chen, LX ; Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China.; Chen, LX (reprint author), Yantai Univ, Coll Life Sci, Yantai 264005, Peoples R China. fbyu@yic.ac.cn; lxchen@yic.ac.cn
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| 发表期刊 | ANALYTICAL CHEMISTRY
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| ISSN | 0003-2700 |
| 2016-04-05 | |
| 卷号 | 88期号:7页码:4122-4129 |
| DOI | 10.1021/acs.analchem.6b00458 |
| 产权排序 | [Huang, Yan; Yu, Fabiao; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Huang, Yan; Wang, Jianchao] Qinghai Normal Univ, Dept Chem, Xining 810008, Peoples R China; [Chen, Lingxin] Yantai Univ, Coll Life Sci, Yantai 264005, Peoples R China |
| 作者部门 | 中科院海岸带环境过程与生态修复重点实验室 |
| 英文摘要 | H2S plays important physiological and pathological roles in the cardiovascular system and nervous system. However, recent evidence imply that hydrogen polysulfides (H2Sn) are the actual signaling molecules in cells. Although H2Sn have been demonstrated to be responsible for mediating tumor suppressors, ion channels, and transcription factors, more of their biological effects are still need to be elaborated. On one hand, H2S have been suggested to be generated from endogenous H2S upon reaction with reactive oxygen species (ROS). On the other hand, H2S derivatives are proposed to be a kind of direct antioxidant against intracellular oxidative stress. This conflicting results should be attributed to the regulation of redox homeostasis between ROS and H2Sn. Superoxide anion (O-2(center dot-) is undoubtedly the primary ROS existing in mitochondria. We reason that the balance of O-2(center dot-) and H2S are pivotal in physiological and pathological processes. Herein, we report two near-infrared fluorescent probes Hcy-Mito and Hcy-Biot for the detection of O-2(center dot-) and H2S, in cells and in vivo. Hcy-Mito is conceived to be applied in mitochondria, and Hcy-Biot is designed to target tumor tissue. Both of the probes were successfully applied for visualizing exogenous and endogenous O-2(center dot-) and H2Sn in living cells and in tumor mice models. The results demonstrate that H2Sn can be promptly produced by mitochondrial oxidative stress. Flow cytometry assays for apoptosis suggest that H2Sn play critical roles in antioxidant systems. |
| 文章类型 | Article |
| 资助机构 | National Nature Science Foundation of China (NSFC)(21405172 ; program of Youth Innovation Promotion Association, CAS(2015170) ; 21575159 ; 21275158) |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | REACTIVE OXYGEN ; LIVING CELLS ; HYPOCHLOROUS ACID ; CHEMICAL BIOLOGY ; SULFANE SULFUR ; SULFIDE ; VIVO ; PEROXYNITRITE ; H2S ; LIPOPOLYSACCHARIDE |
| 研究领域[WOS] | Chemistry |
| WOS记录号 | WOS:000373656300093 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/17035 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室 |
| 通讯作者 | Yu, FB; Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China.; Chen, LX (reprint author), Yantai Univ, Coll Life Sci, Yantai 264005, Peoples R China. fbyu@yic.ac.cn; lxchen@yic.ac.cn |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat 2.Qinghai Normal Univ, Dept Chem 3.Yantai Univ, Coll Life Sci |
| 推荐引用方式 GB/T 7714 | Huang, Y,Yu, FB,Wang, JC,et al. Near-Infrared Fluorescence Probe for in Situ Detection of Superoxide Anion and Hydrogen Polysulfides in Mitochondrial Oxidative Stress[J]. ANALYTICAL CHEMISTRY,2016,88(7):4122-4129. |
| APA | Huang, Y.,Yu, FB.,Wang, JC.,Chen, LX.,Chen, LX .,...&lxchen@yic.ac.cn.(2016).Near-Infrared Fluorescence Probe for in Situ Detection of Superoxide Anion and Hydrogen Polysulfides in Mitochondrial Oxidative Stress.ANALYTICAL CHEMISTRY,88(7),4122-4129. |
| MLA | Huang, Y,et al."Near-Infrared Fluorescence Probe for in Situ Detection of Superoxide Anion and Hydrogen Polysulfides in Mitochondrial Oxidative Stress".ANALYTICAL CHEMISTRY 88.7(2016):4122-4129. |
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