A SERS-based lateral flow assay biosensor for highly sensitive detection of HIV-1 DNA

doi:10.1016/j.bios.2015.11.099

	A SERS-based lateral flow assay biosensor for highly sensitive detection of HIV-1 DNA
	Fu, X ; Cheng, Z ; Yu, J ; Choo, P ; Chen, L ; Choo, J ; Choo, J (reprint author), Hanyang Univ, Dept Bionano Technol, Ansan 426791, South Korea. jbchoo@hanyang.ac.kr
发表期刊	BIOSENSORS & BIOELECTRONICS
ISSN	0956-5663
	2016-04-15
卷号	78 页码:530-537
关键词	Human Immunodeficiency Virus Type 1 (Hiv-1) Surface-enhanced Raman Scattering Gold Nanoparticles Dna Lateral Flow Assay
DOI	10.1016/j.bios.2015.11.099
产权排序	[Fu, Xiuli; Cheng, Ziyi; Yu, Jimin; Choo, Jaebum] Hanyang Univ, Dept Bionano Technol, Ansan 426791, South Korea; [Choo, Priscilla] Indiana Univ, Dept Chem, Bloomington, IN 47405 USA; [Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
作者部门	中科院海岸带环境过程与生态修复重点实验室
英文摘要	User-friendly lateral flow (LF) strips have been extensively used for point-of-care (POC) self-diagnostics, but they have some limitations in their detection sensitivity and quantitative analysis because they only identify the high cut-off value of a biomarker by utilizing color changes that are detected with the naked eye. To resolve these problems associated with LF strips, we developed a novel surface-enhanced Raman scattering (SERS)-based LF assay for the quantitative analysis of a specific biomarker in the low concentration range. Herein, human immunodeficiency virus type 1 (HIV-1) DNA was chosen as the specific biomarker. Raman reporter-labeled gold nanoparticles (AuNPs) were employed as SERS nano tags for targeting and detecting the HIV-1 DNA marker, as opposed to using bare AuNPs in LF strips. It was possible to quantitatively analyze HIV-1 DNA with high sensitivity by monitoring the characteristic Raman peak intensity of the DNA-conjugated AuNPs. Under optimized conditions, the detection limit of our SERS-based lateral flow assay was 0.24 pg/mL, which was at least 1000 times more sensitive compared to colorimetric or fluorescent detection methods. These results demonstrate the potential feasibility of the proposed SERS-based lateral flow assay to quantitatively detect a broad range of genetic diseases with high sensitivity. (C) 2015 Elsevier B.V. All rights reserved.
文章类型	Article
资助机构	National Research Foundation of Korea(2008-0061891 ; Nano Material Technology Development Program through the National Research Foundation of Korea ; Ministry of Science, ICT and Future Planning(2012M3A7B4035288) ; National Natural Science Foundation of China(21275158) ; 2009-00426)
收录类别	SCI
语种	英语
关键词[WOS]	SURFACE-ENHANCED RAMAN ; FUNCTIONALIZED GOLD NANOPARTICLES ; DETECTION PLATFORM ; VISUAL DETECTION ; STRIP BIOSENSOR ; IMMUNOASSAY ; SCATTERING ; ELISA ; SPECTROSCOPY ; SILVER
研究领域[WOS]	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS记录号	WOS:000368954900074
引用统计	被引频次：279[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/10874
专题	中国科学院海岸带环境过程与生态修复重点实验室
通讯作者	Choo, J (reprint author), Hanyang Univ, Dept Bionano Technol, Ansan 426791, South Korea. jbchoo@hanyang.ac.kr
作者单位	1.Hanyang Univ, Dept Bionano Technol 2.Indiana Univ, Dept Chem 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat
推荐引用方式 GB/T 7714	Fu, X,Cheng, Z,Yu, J,et al. A SERS-based lateral flow assay biosensor for highly sensitive detection of HIV-1 DNA[J]. BIOSENSORS & BIOELECTRONICS,2016,78:530-537.
APA	Fu, X.,Cheng, Z.,Yu, J.,Choo, P.,Chen, L.,...&Choo, J .(2016).A SERS-based lateral flow assay biosensor for highly sensitive detection of HIV-1 DNA.BIOSENSORS & BIOELECTRONICS,78,530-537.
MLA	Fu, X,et al."A SERS-based lateral flow assay biosensor for highly sensitive detection of HIV-1 DNA".BIOSENSORS & BIOELECTRONICS 78(2016):530-537.

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