Purification and Characterization of Bioactive Compounds from Styela clava
Ju, Bao1; Chen, Bin2; Zhang, Xingrong1; Han, Chunling1; Jiang, Aili1; Jiang, AL (reprint author), Yantai Univ, Dept Bioengn, Yantai 264005, Shandong, Peoples R China. jal9035@live.cn
发表期刊JOURNAL OF CHEMISTRY
ISSN2090-9063
2014
页码525141
关键词Didemnin-b Marine Extracts Tunicate Agent
DOI10.1155/2014/525141
产权排序[Ju, Bao; Zhang, Xingrong; Han, Chunling; Jiang, Aili] Yantai Univ, Dept Bioengn, Yantai 264005, Shandong, Peoples R China; [Chen, Bin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China
作者部门中科院烟台海岸带研究所知识产出
英文摘要The immunomodulatory activity of extract from Styela clava was studied systematically based on activity tracking in vitro in order to find out novel-structured secondary metabolite. The proliferation rates of mouse splenic lymphocytes and peritoneal macrophages were used as screening index, as well as NO release promoting activities. The crude extract (CE) and its different polar fractions from S. clava all exhibited proliferative activity of splenolymphocytes and mouse macrophages, as well as NO release promoting activities, among which petroleum ether fraction (PE) showed the strongest effect. The antioxidant experiment in vitro showed that CE demonstrated antioxidant ability in 1,1-diphenyl-2-picrylhydrazyl (DPPH) system and the beta carotene-linoleic acid system; the activity of ethyl acetate fraction (ET) was much stronger than that of the others. Further isolated by silica gel column chromatography, ET was classified into seven sub-components (E1 similar to E7) listed in the order of activity as E5 > E6 > E4 > E3 > E7 > E2 > E1. Five compounds were separated as (1) cholesteric-7-en-3 beta-ol, (2) cholesteric-4-en-3 beta,6 beta-diol, (3) cholesterol, (4) batilol, and (5) ceramide, among which (1), (2), and (4) were isolated for the first time from S. clava.; The immunomodulatory activity of extract from Styela clava was studied systematically based on activity tracking in vitro in order to find out novel-structured secondary metabolite. The proliferation rates of mouse splenic lymphocytes and peritoneal macrophages were used as screening index, as well as NO release promoting activities. The crude extract (CE) and its different polar fractions from S. clava all exhibited proliferative activity of splenolymphocytes and mouse macrophages, as well as NO release promoting activities, among which petroleum ether fraction (PE) showed the strongest effect. The antioxidant experiment in vitro showed that CE demonstrated antioxidant ability in 1,1-diphenyl-2-picrylhydrazyl (DPPH) system and the beta carotene-linoleic acid system; the activity of ethyl acetate fraction (ET) was much stronger than that of the others. Further isolated by silica gel column chromatography, ET was classified into seven sub-components (E1 similar to E7) listed in the order of activity as E5 > E6 > E4 > E3 > E7 > E2 > E1. Five compounds were separated as (1) cholesteric-7-en-3 beta-ol, (2) cholesteric-4-en-3 beta,6 beta-diol, (3) cholesterol, (4) batilol, and (5) ceramide, among which (1), (2), and (4) were isolated for the first time from S. clava.
文章类型Article
资助机构Chemistry
收录类别SCI
语种英语
关键词[WOS]DIDEMNIN-B ; MARINE ; EXTRACTS ; TUNICATE ; AGENT
研究领域[WOS]Chemistry
WOS记录号WOS:000335774000001
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/8805
专题中国科学院烟台海岸带研究所知识产出
通讯作者Jiang, AL (reprint author), Yantai Univ, Dept Bioengn, Yantai 264005, Shandong, Peoples R China. jal9035@live.cn
作者单位1.Yantai Univ, Dept Bioengn, Yantai 264005, Shandong, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China
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Ju, Bao,Chen, Bin,Zhang, Xingrong,et al. Purification and Characterization of Bioactive Compounds from Styela clava[J]. JOURNAL OF CHEMISTRY,2014:525141.
APA Ju, Bao,Chen, Bin,Zhang, Xingrong,Han, Chunling,Jiang, Aili,&Jiang, AL .(2014).Purification and Characterization of Bioactive Compounds from Styela clava.JOURNAL OF CHEMISTRY,525141.
MLA Ju, Bao,et al."Purification and Characterization of Bioactive Compounds from Styela clava".JOURNAL OF CHEMISTRY (2014):525141.
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