YIC-IR  > 中科院烟台海岸带研究所知识产出
大竹蛏(Solen grandis)翻译控制肿瘤蛋白(TCTP)基因的分子克隆和表达特征分析
Alternative TitleMOLECULAR CLONING AND EXPRESSION ANALYSIS OF A TRANSLATIONALLY CONTROLLED TUMOR PROTEIN (TCTP) GENE FROM SOLEN GRANDIS
韦秀梅 ; 杨顶珑 ; 杨建敏 ; 杨嘉龙 ; 徐洁 ; 刘相全 ; 张艳敏
2013-05-15
Source Publication海洋与湖沼
ISSN0029-814X
Volume44Issue:3Pages:584-589
Contribution Rank山东省海洋水产研究所山东省海洋生态修复重点实验室;中国科学院烟台海岸带研究所;山东商务职业学院;
Abstract本研究克隆得到了一个大竹蛏(Solen grandis)翻译控制肿瘤蛋白(TCTP)基因(SgTCTP)的cDNA全长,其序列全长为1055bp,5′和3′端的非编码区(UTR)分别为54和461bp,开放阅读框(ORF)540bp,编码179个氨基酸,理论等电点为4.50,预测分子量大小19.96kDa。通过荧光定量PCR法检测了SgTCTP在健康大竹蛏各组织中和病原相关分子模式(PAMPs)刺激后的表达规律,结果表明:SgTCTP在检测组织外套膜、鳃、性腺、血细胞、肌肉和肝胰腺中都有表达,其中在肝胰腺中的表达量最高。脂多糖(LPS)、肽聚糖(PGN)和葡聚糖(β-1,3-glucan)刺激...
Corresponding Author刘相全 E-mail:lxq6808@163.com
Other AbstractIn the present study, a translationally controlled tumor protein (TCTP) gene was identified from Solen grandis (designated as SgTCTP). The full-length cDNA of SgTCTP was of 1055bp, containing a 5’ untranslated region (UTR) of 54bp, a 3’ UTR of 461bp with a poly (A) tail, and an open reading frame (ORF) of 537bp encoding a polypeptide of 179 amino acids with the predicted molecular weight of 19.96kDa. The expression patterns both in tissues and towards pathogen associated molecular patterns (PAMPs) stimulation were then characterized by real-time PCR. SgTCTP was constitutively expressed in all tested tissues, including mantle, gill, gonad, hemocyte, muscle, and hepatopancreas, and it was highly expressed in hepatopancreas. The mRNA expression of SgTCTP could be induced by stimulation of β-1,3-glucan, LPS and PGN. The expression level of SgTCTP reached the peak at 12h and 3h post LPS and PGN stimulation, respectively. After β-1,3-glucan stimulation, SgTCTP expression reached the maximal level at 12h post stimulation, which was 11.76-fold compared with the blank group. All these results indicated that SgTCTP was an acute-phase protein involved in the immune response of S. grandis.
Keyword大竹蛏 翻译控制肿瘤蛋白 免疫应答 荧光定量pcr
Department中科院烟台海岸带研究所知识产出
Subject Area分子生物学
Funding Organization山东省农业良种工程课题“优质高产抗逆贝类良种选育”,2009—2013;; 国家自然科学基金项目资助,31202025号;; 水生动物营养与饲料“泰山学者”岗位经费资助
Indexed ByCSCD
Language中文
CSCD IDCSCD:4875607
Citation statistics
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/6690
Collection中科院烟台海岸带研究所知识产出
Recommended Citation
GB/T 7714
韦秀梅,杨顶珑,杨建敏,等. 大竹蛏(Solen grandis)翻译控制肿瘤蛋白(TCTP)基因的分子克隆和表达特征分析[J]. 海洋与湖沼,2013,44(3):584-589.
APA 韦秀梅.,杨顶珑.,杨建敏.,杨嘉龙.,徐洁.,...&张艳敏.(2013).大竹蛏(Solen grandis)翻译控制肿瘤蛋白(TCTP)基因的分子克隆和表达特征分析.海洋与湖沼,44(3),584-589.
MLA 韦秀梅,et al."大竹蛏(Solen grandis)翻译控制肿瘤蛋白(TCTP)基因的分子克隆和表达特征分析".海洋与湖沼 44.3(2013):584-589.
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