This study aimed to extract antibacterial peptides from mussel.[Method] Blue mussels were used as raw materials for direct extraction of antibacterial peptides by using 0.5% acetic acid,and the antibacterial peptides were isolated and purified by Sephacryl S-100 polyacrylamide gel chromatography.The fractions were collected for determination of antibacterial activity and minimum inhibitory concentration(MIC) for a variety of bacterial species by filter paper diffusion assay.Molecular weight of the antibacterial peptides was determined by SDS-PAGE.Variation of antibacterial activity of antibacterial peptides was measured at 100 ℃ under conditions of different processing time and different pH.[Result] 0.5% acetic acid was used for crude extraction of antimicrobial peptides as extraction solution and led to relatively high extraction efficiency.By using Sephacryl S-100,the antimicrobial peptides could be purified as a single substance.The isolated and purified antibacterial peptides of mussel had relatively strong antimicrobial properties with molecular weight of 5 908,showing heat-resistance acid-alkaline resistance.[Conclusion] This study had laid the theoretical foundation for large-scale production of antimicrobial peptides.
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