Study on Separation of Phycoerythrin by Q-Sepharose Fast Flow

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	Study on Separation of Phycoerythrin by Q-Sepharose Fast Flow
	Zhang B(张彬) ; Wang CQ(王翠芹) ; Gao M(高敏) ; Zhang Y(张轶) ; Gu M(顾铭) ; Wang ZH(王长海)
发表期刊	Agricultural Science & Technology
ISSN	1009-4229
	2012-09-01
期号	8 页码:1641-1644
关键词	离子交换色谱 q Sepharose Ff 藻红蛋白分离工艺放大
产权排序	中国科学院烟台海岸带研究所;中国科学院研究生院;中国科学院过程工程研究所;南京农业大学;
通讯作者	顾铭 guming@home.ipe.ac.cn
作者部门	分析测试中心
英文摘要	This study aimed to establish an efficient process for separation of phycoerythrin by using Q Sepharose Fast Flow resin and verify its feasibility.[Method] Elution gradient,loading volume and flow rate were optimized to determine the optimal separation condition,under which the scale up process was verified.[Result] The optimal condition for separation of phycoerythrin by using Q Sepharose Fast Flow resin was investigated： 30 ml of laver extract was loaded to a Q Sepharose Fast Flow column with a bed volume of 8 ml;subsequently,the column was eluted under a stepwise gradient elution of 0-0.10-0.35-1.00 mol/L NaCl solution（pH 6.0） at a constant flow rate of 1 ml/min;the elution peak under 0.35 mol/L NaCl solution was collected,and the recovery rate and purity coefficient（A565/A280） of phycoerythrin were determined as 44.3 and 1.15,respectively.Based on the established process,75 ml of phycoerythrin extract was loaded to a Q Sepharose Fast Flow column with bed volume of 20 ml for separation,while no significant variation was observed in separation result.[Conclusion] Phycoerythrin can be well separated from laver extract by Q Sepharose Fast Flow resin and the process is feasible for scale-up.
中文摘要	[目的]建立Q Sepharose FF分离藻红蛋白的工艺方法并验证其放大可行性。[方法]通过对洗脱条件、上样体积以及洗脱流速的优化;确定最佳分离工艺条件;在确定的工艺条件下;将上样体积与柱体积等比例放大。[结果]Q Sepharose FF分离藻红蛋白的最佳工艺条件为:将30ml坛紫菜提取液加载到8ml Q Sepharose FF柱上;以1ml/min的流速使用添加0-0.10-0.35-1.00mol/LNaCl的NaH2PO4-Na2HPO4缓冲溶液(pH6.0)分步洗脱;收集0.35mol/LNaCl溶液洗脱时的色谱峰。在些条件下;R-藻红蛋白的回收率和纯度系数(A565/A280)分别为44.3和1.15。按照该工艺;将75ml藻红蛋白提取液加载到20ml Q Sepharose FF柱上进行分离;发现分离效果没有发生明显变化。[结论]使用Q Sepharose FF介质能够分离坛紫菜提取液中的藻红蛋白且该工艺易于放大。
资助机构	Supported by National Natural Science Foundation of China(51143012);Natural Science Foundation of Shandong Province(ZR2009BM006)~~
语种	中文
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/5906
专题	支撑部门
推荐引用方式 GB/T 7714	Zhang B,Wang CQ,Gao M,et al. Study on Separation of Phycoerythrin by Q-Sepharose Fast Flow[J]. Agricultural Science & Technology,2012(8):1641-1644.
APA	Zhang B,Wang CQ,Gao M,Zhang Y,Gu M,&Wang ZH.(2012).Study on Separation of Phycoerythrin by Q-Sepharose Fast Flow.Agricultural Science & Technology(8),1641-1644.
MLA	Zhang B,et al."Study on Separation of Phycoerythrin by Q-Sepharose Fast Flow".Agricultural Science & Technology .8(2012):1641-1644.

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