3、通过将乙醇胺嫁接到罗丹明B基体中，自主设计合成了一类新型的Fe³⁺荧光探针。详细考查了其对不同金属离子的光谱分辨能力，结果表明：在中性乙醇–水溶液中，探针只对Fe³⁺表现出选择性的荧光增强和颜色变化（从无色到粉色），检测限达到10^-7 M的水平，这一变化是源于从内酰胺环（无荧光/off型）到开环胺（荧光/on型）的转化过程，对其它金属离子没有反应。此外共聚焦扫描显微镜实验表明，该探针具有较好的细胞渗透性，可以分析检测活细胞中的Fe³⁺。

本章主要是对其它一些体系的研究，希望对其他科研工作者有所帮助和启示。

Fluorescent probes can transfer molecular recognition events into fluorescence signals and then make a bridge between human and molecule. They have increasingly been important due to their distinct characteristics in exploring the composition, structure and evolution of environmental or biological microsystems. Since the extensively applications of rhodamine B and naphthalimide-based fluorescent probes in biological and environmental samples, it is necessary and argent to develop more kinds of such fluorescent probes to meet the increasing demands. Some fluorescent probes were developed in this dissertation based on a rhodamine B and naphthalimide derivatives, where some metal ions including Cu²⁺, Fe³⁺, Ag⁺, Zn²⁺ as sensing targets. This dissertation consisted of seven chapters summarized as follows.

In chapter 1, a general introduction to fluorescent probes was presented. Emphasis was paid on the new developments of fluorescent probes, especially those based on rhaodamine B and naphthalimide derivatives owing to their excellent properties in photochemistry and photophysics. Based on reviewing recent progress of rhaodamine B and naphthalimide derivatives as fluorescent probes, the objective of this dissertation was presented.

In chapter 5, a Schiff-base probe for Zn²⁺ derived from salicylide and 2-amino benzoyl hydrazine motif introduced to naphthalimide core was designed and synthesized. The fluorescent probe exhibited its selectivity to Zn²⁺ among a series of metal ions in ethanol. The “off-on” enhancement and slight res shift of fluorescence of probe caused by Zn²⁺ was due to restricting the rotation of C=N bonds, although other mechanisms such as ESIPT (Excited-State Intramolecular Proton Transfer) process and the chelate effect in the complex of Zn²⁺-probe may be helpful to contribute to this changes.

In chapter 7, some other studies were explored to provide references for improvement of the system of fluorescent probes.