Recognition of DNA by designed ligands at subnanomolar concentrations

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	Recognition of DNA by designed ligands at subnanomolar concentrations
	Trauger JW ; Baird EE ; Dervan PB
发表期刊	Nature
	1996
卷号	382 页码:559-561
通讯作者	Dervan PB
英文摘要	Small molecules that specifically bind with high affinity to any predetermined DNA sequence in the human genome would be useful tools in molecular biology and potentially in human medicine. Simple rules have been developed to control rationally the sequence specificity of minor-groove-binding polyamides containing N-methylimidazole and N-methylpyrrole amino acids. Two eight-ring pyrrole-imidazole polyamides differing in sequence by a single amino acid bind specifically to respective six-base-pair target sites which differ in sequence by a single base pair. Binding is observed at subnanomolar concentrations of ligand. The replacement of a single nitrogen atom with a C-H regulates affinity and specificity by two orders of magnitude. The broad range of sequences that can be specifically targeted with pyrrole-imidazole polyamides, coupled with an efficient solid-phase synthesis methodology, identify a powerful class of small molecules for sequence-specific recognition of double-helical DNA.
语种	英语
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/4619
专题	科研共享资源
推荐引用方式 GB/T 7714	Trauger JW,Baird EE,Dervan PB. Recognition of DNA by designed ligands at subnanomolar concentrations[J]. Nature,1996,382:559-561.
APA	Trauger JW,Baird EE,&Dervan PB.(1996).Recognition of DNA by designed ligands at subnanomolar concentrations.Nature,382,559-561.
MLA	Trauger JW,et al."Recognition of DNA by designed ligands at subnanomolar concentrations".Nature 382(1996):559-561.

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