Analysis of Atrazine in Water and Vegetables Using Immunosensors Working in Organic Media | |
Gonzalez-Martinez MA ; Maquieira A ; Puchades R | |
发表期刊 | International Journal of Environmental and Analytical Chemistry
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2003 | |
卷号 | 83期号:7-8页码:633-642 |
关键词 | Organic Solvents Immunosensor Pesticides Water Vegetables |
通讯作者 | Puchades R |
英文摘要 | Flow immunosensors using a competitive capture format have been studied and applied to the analysis of atrazine in extracts of water and vegetables containing high percentages of organic solvents. Four organic mixtures have been assayed: M1 50% methanol-50% buffer, M2 25% isopropanol-25% methanol-50% buffer, M3 25% acetonitrile-75% buffer, and M4 10% ethyl acetate-25% methanol-65% buffer. Three polyclonal antisera and two haptens conjugated to horseradish peroxidase and alkaline phosphatase as enzyme tracers have been studied with each mixture. Although sensitivity is better in aqueous medium, good results have been obtained with the four organic mixtures tested, being best in M1, with a limit of detection of 0.15 mu g L super(-1) for the sensor employing peroxidase as label. Selectivity, expressed as cross-reactivity, and precision of the assays have been shown to be better in organic media than in aqueous one. More than 400 assay cycles can be run with the same immunosensor. Good recoveries have been obtained when the methanolic extracts of atrazine-spiked water and vegetables were analysed after solid phase extraction on C sub(18) cartridges. These results show the potential of organic immunoassays. |
语种 | 英语 |
文献类型 | 期刊论文 |
条目标识符 | http://ir.yic.ac.cn/handle/133337/4463 |
专题 | 科研共享资源 |
推荐引用方式 GB/T 7714 | Gonzalez-Martinez MA,Maquieira A,Puchades R. Analysis of Atrazine in Water and Vegetables Using Immunosensors Working in Organic Media[J]. International Journal of Environmental and Analytical Chemistry,2003,83(7-8):633-642. |
APA | Gonzalez-Martinez MA,Maquieira A,&Puchades R.(2003).Analysis of Atrazine in Water and Vegetables Using Immunosensors Working in Organic Media.International Journal of Environmental and Analytical Chemistry,83(7-8),633-642. |
MLA | Gonzalez-Martinez MA,et al."Analysis of Atrazine in Water and Vegetables Using Immunosensors Working in Organic Media".International Journal of Environmental and Analytical Chemistry 83.7-8(2003):633-642. |
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