Analysis of Atrazine in Water and Vegetables Using Immunosensors Working in Organic Media

YIC-IR > 科研共享资源

	Analysis of Atrazine in Water and Vegetables Using Immunosensors Working in Organic Media
	Gonzalez-Martinez MA ; Maquieira A ; Puchades R
发表期刊	International Journal of Environmental and Analytical Chemistry
	2003
卷号	83 期号:7-8 页码:633-642
关键词	Organic Solvents Immunosensor Pesticides Water Vegetables
通讯作者	Puchades R
英文摘要	Flow immunosensors using a competitive capture format have been studied and applied to the analysis of atrazine in extracts of water and vegetables containing high percentages of organic solvents. Four organic mixtures have been assayed: M1 50% methanol-50% buffer, M2 25% isopropanol-25% methanol-50% buffer, M3 25% acetonitrile-75% buffer, and M4 10% ethyl acetate-25% methanol-65% buffer. Three polyclonal antisera and two haptens conjugated to horseradish peroxidase and alkaline phosphatase as enzyme tracers have been studied with each mixture. Although sensitivity is better in aqueous medium, good results have been obtained with the four organic mixtures tested, being best in M1, with a limit of detection of 0.15 mu g L super(-1) for the sensor employing peroxidase as label. Selectivity, expressed as cross-reactivity, and precision of the assays have been shown to be better in organic media than in aqueous one. More than 400 assay cycles can be run with the same immunosensor. Good recoveries have been obtained when the methanolic extracts of atrazine-spiked water and vegetables were analysed after solid phase extraction on C sub(18) cartridges. These results show the potential of organic immunoassays.
语种	英语
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/4463
专题	科研共享资源
推荐引用方式 GB/T 7714	Gonzalez-Martinez MA,Maquieira A,Puchades R. Analysis of Atrazine in Water and Vegetables Using Immunosensors Working in Organic Media[J]. International Journal of Environmental and Analytical Chemistry,2003,83(7-8):633-642.
APA	Gonzalez-Martinez MA,Maquieira A,&Puchades R.(2003).Analysis of Atrazine in Water and Vegetables Using Immunosensors Working in Organic Media.International Journal of Environmental and Analytical Chemistry,83(7-8),633-642.
MLA	Gonzalez-Martinez MA,et al."Analysis of Atrazine in Water and Vegetables Using Immunosensors Working in Organic Media".International Journal of Environmental and Analytical Chemistry 83.7-8(2003):633-642.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Analysis of Atrazine（4376KB）			限制开放	--	请求全文