Infection-stimulated Anemia Results Primarily from Interferon Gamma-dependent, Signal Transducer and Activator of Transcription 1-independent Red Cell Loss

YIC-IR

	Infection-stimulated Anemia Results Primarily from Interferon Gamma-dependent, Signal Transducer and Activator of Transcription 1-independent Red Cell Loss
其他题名	Infection-stimulated Anemia Results Primarily from Interferon Gamma-dependent, Signal Transducer and Activator of Transcription 1-independent Red Cell Loss
	Wang Zheng 1; Zhang DongXia 2; Zhao Qi 2
发表期刊	CHINESE MEDICAL JOURNAL
ISSN	0366-6999
	2015
卷号	128 期号:7 页码:948-955
关键词	TOXOPLASMA-GONDII INFECTION TUMOR-NECROSIS-FACTOR IFN-GAMMA NITRIC-OXIDE FACTOR-ALPHA TNF-ALPHA STAT1-INDEPENDENT PATHWAYS EFFECTOR MECHANISMS INDUCIBLE PROTEIN VIRUS-INFECTION Anemia Hemorrhage Signal Transducer and Activator of Transcription 1 Toxoplasma gondii
英文摘要	Background: Although the onset of anemia during infectious disease is commonly correlated with production of inflammatory cytokines, the mechanisms by which cytokines induce anemia are poorly defined. This study focused on the mechanism research.
中文摘要	Background: Although the onset of anemia during infectious disease is commonly correlated with production of inflammatory cytokines, the mechanisms by which cytokines induce anemia are poorly defined. This study focused on the mechanism research. Methods: Different types of mice were infected perorally with Toxoplasma gondii strain ME49. At the indicated times, samples from each mouse were harvested, processed, and analyzed individually. Blood samples were analyzed using a Coulter Counter and red blood cell (RBC) survival was measured by biotinylation. Levels of tumor necrosis factor-α (TNF-α), inducible nitric oxide synthase (iNOS), and inducible protein 10 (IP-10) mRNA in liver tissue were measured by real-time polymerase chain reaction. Results: T. gondii -infected mice exhibited anemia due to a decrease in both erythropoiesis and survival time of RBC in the circulation ( P < 0.02). In addition, infection-stimulated anemia was associated with fecal occult, supporting previous literature that hemorrhage is a consequence of T. gondii infection in mice. Infection-induced anemia was abolished in interferon gamma (IFNγ) and IFNγ receptor deficient mice ( P < 0.05) but was still evident in mice lacking TNF-α, iNOS, phagocyte NADPH oxidase or IP-10 ( P < 0.02). Neither signal transducer and activator of transcription 1 (STAT1) deficient mice nor 129S6 controls exhibited decreased erythropoiesis, but rather suffered from an anemia resulting solely from increased loss of circulating RBC. Conclusions: Infection-stimulated decrease in erythropoiesis and losses of RBC have distinct mechanistic bases. These results show that during T. gondii infection, IFNγ is responsible for an anemia that results from both a decrease in erythropoiesis and a STAT1 independent loss of circulating RBC.
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:5392559
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/35108
专题	中国科学院烟台海岸带研究所
作者单位	1.Gen Hosp Chinese Peoples Liberat Army, Department Pediat Surg, Beijing 100037, Peoples R China Department Pediat Surg 2.中国科学院烟台海岸带研究所
推荐引用方式 GB/T 7714	Wang Zheng,Zhang DongXia,Zhao Qi. Infection-stimulated Anemia Results Primarily from Interferon Gamma-dependent, Signal Transducer and Activator of Transcription 1-independent Red Cell Loss[J]. CHINESE MEDICAL JOURNAL,2015,128(7):948-955.
APA	Wang Zheng,Zhang DongXia,&Zhao Qi.(2015).Infection-stimulated Anemia Results Primarily from Interferon Gamma-dependent, Signal Transducer and Activator of Transcription 1-independent Red Cell Loss.CHINESE MEDICAL JOURNAL,128(7),948-955.
MLA	Wang Zheng,et al."Infection-stimulated Anemia Results Primarily from Interferon Gamma-dependent, Signal Transducer and Activator of Transcription 1-independent Red Cell Loss".CHINESE MEDICAL JOURNAL 128.7(2015):948-955.