Marine Bacterial Biofilms in Bioremediation of Polycyclic Aromatic Hydrocarbons (PAHs) Under Terrestrial Condition in a Soil Microcosm

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	Marine Bacterial Biofilms in Bioremediation of Polycyclic Aromatic Hydrocarbons (PAHs) Under Terrestrial Condition in a Soil Microcosm
其他题名	Marine Bacterial Biofilms in Bioremediation of Polycyclic Aromatic Hydrocarbons (PAHs) Under Terrestrial Condition in a Soil Microcosm
	Mangwani Neelam; Kumari Supriya; Das Surajit
发表期刊	PEDOSPHERE
ISSN	1002-0160
	2017
卷号	27 期号:3 页码:548-558
关键词	PHENANTHRENE DEGRADATION NAPHTHALENE DEGRADATION PSEUDOMONAS-AERUGINOSA MICROBIAL CONSORTIA META-CLEAVAGE BIODEGRADATION PYRENE BIOAVAILABILITY SYSTEM POLLUTANTS catechol 2,3-dioxygenase activity chemotactic movement degradation extracellular polymeric substances pyrene phenanthrene
英文摘要	Polycyclic aromatic hydrocarbons (PAHs) in soil retain for a quite long period due to their hydrophobicity and aggregation properties. Biofilm-forming marine bacterial consortium (named as NCPR), composed of Stenotrophomonas acidaminiphila NCW702, Alcaligenes faecalis NCW402, Pseudomonas mendocina NR802, Pseudomonas aeruginosa N6P6, and Pseudomonas pseudoalcaligenes NP103, was used for the bioremediation of PAHs in a soil microcosm. Phenanthrene and pyrene were used as reference PAHs. Parameters that can affect PAH degradation, such as chemotaxis, solubility of PAHs in extracellular polymeric substances (EPS), and catechol 2,3-dioxygenase (C23O) activity, were evaluated. P. aeruginosa N6P6 and P. pseudoalcaligenes NP103 showed chemotactic movement towards both the reference PAHs. The solubility of both the PAHs was increased with an increase in EPS concentration (extracted from all the 5 selected isolates). Significantly (P < 0.001) high phenanthrene (70.29%) and pyrene (55.54%) degradation was observed in the bioaugmented soil microcosm. The C230 enzyme activity was significantly (P < 0.05) higher in the bioaugmented soil microcosm with phenanthrene added at 173.26 +/- 2.06 nmol min(-1) mg(-1) protein than with pyrene added at 61.80 +/- 2.20 nmol min(-1) mg(-1) protein. The C23O activity and gas chromatography-mass spectrometer analyses indicated catechol pathway of phenanthrene metabolism. However, the metabolites obtained from the soil microcosm added with pyrene revealed both the catechol and phthalate pathways for pyrene degradation.
中文摘要	Polycyclic aromatic hydrocarbons (PAHs) in soil retain for a quite long period due to their hydrophobicity and aggregation properties. Biofilm-forming marine bacterial consortium (named as NCPR), composed of Stenotrophomonas acidaminiphila NCW702, Alcaligenes faecalis NCW402, Pseudomonas mendocina NR802, Pseudomonas aeruginosa N6P6, and Pseudomonas pseudoalcaligenes NP103, was used for the bioremediation of PAHs in a soil microcosm. Phenanthrene and pyrene were used as reference PAHs. Parameters that can affect PAH degradation, such as chemotaxis, solubility of PAHs in extracellular polymeric substances (EPS), and catechol 2,3-dioxygenase (C230) activity, were evaluated. P. aeruginosa N6P6 and P. pseudoalcaligenes NP103 showed chemotactic movement towards both the reference PAHs. The solubility of both the PAHs was increased with an increase in EPS concentration (extracted from all the 5 selected isolates). Significantly (P < 0.001) high phenanthrene (70.29%) and pyrene (55.54%) degradation was observed in the bioaugmented soil microcosm. The C230 enzyme activity was significantly (P < 0.05) higher in the bioaugmented soil microcosm with phenanthrene added at 173.26 ± 2.06 nmol min~(-1) mg~(-1) protein than with pyrene added at 61.80 ± 2.20 nmol min~(-1) mg~(-1) protein. The C230 activity and gas chromatography-mass spectrometer analyses indicated catechol pathway of phenanthrene metabolism. However, the metabolites obtained from the soil microcosm added with pyrene revealed both the catechol and phthalate pathways for pyrene degradation.
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:6007812
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/35036
专题	中国科学院烟台海岸带研究所
作者单位	中国科学院烟台海岸带研究所
推荐引用方式 GB/T 7714	Mangwani Neelam,Kumari Supriya,Das Surajit. Marine Bacterial Biofilms in Bioremediation of Polycyclic Aromatic Hydrocarbons (PAHs) Under Terrestrial Condition in a Soil Microcosm[J]. PEDOSPHERE,2017,27(3):548-558.
APA	Mangwani Neelam,Kumari Supriya,&Das Surajit.(2017).Marine Bacterial Biofilms in Bioremediation of Polycyclic Aromatic Hydrocarbons (PAHs) Under Terrestrial Condition in a Soil Microcosm.PEDOSPHERE,27(3),548-558.
MLA	Mangwani Neelam,et al."Marine Bacterial Biofilms in Bioremediation of Polycyclic Aromatic Hydrocarbons (PAHs) Under Terrestrial Condition in a Soil Microcosm".PEDOSPHERE 27.3(2017):548-558.