Molecular, serological and biological characterization of a novel Apple stem pitting virus strain from a local pear variety grown in China

YIC-IR

	Molecular, serological and biological characterization of a novel Apple stem pitting virus strain from a local pear variety grown in China
其他题名	Molecular, serological and biological characterization of a novel Apple stem pitting virus strain from a local pear variety grown in China
	LI Liu 1; ZHENG Mengmeng 1; MA Xiaofang 2; LI Yuanjun 3; LI Qingyu 3; WANG Guoping 1; HONG Ni 1
发表期刊	农业科学学报:英文版
ISSN	2095-3119
	2019
卷号	18.0 期号:011 页码:2549-2560
关键词	pear apple stem pitting virus genome Western blot
英文摘要	Apple stem pitting virus(ASPV)is an important causal agent of pear diseases.Nowadays,the infection status and molecular characteristics of the virus in old pear trees have never been investigated.In this study,we provide the first complete genome sequence of an ASPV isolate LYC from an over 300-year-old tree of a local Pyrus bretschneideri cultivar‘Chili’specifically grown at Laiyang area in China.ASPV-LYC possesses a chimeric genome consisting of 9273 nucleotides excluding a poly(A)tail at its 3′end and harboring a recombination region in its open reading frame(ORF1)with Aurora-1 and KL9 identified as the major and minor parents.Western blot analysis with antisera against recombinant coat proteins(CPs)of three ASPV isolates from pear indicates that ASPV-LYC is serologically related to these ASPV isolates,but with differential activities.Further biological tests on indicator plants of Pyronia veitchii show that ASPV-LYC can induce serious leaf and stem symptoms as other ASPV isolates.The results provide an important information for understanding molecular evolution of ASPV and suggest a need to prevent dissemination of the isolate among pear trees.
收录类别	CSCD
语种	中文
CSCD记录号	CSCD:6614244
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/35032
专题	中国科学院烟台海岸带研究所
作者单位	1.State Key Laboratory of Agromicrobiology,Huazhong Agricultural University,P.R.China 2.Key Laboratory of Crop Disease Monitoring and Safety Control in Hubei Province,College of Plant Science and Technology,Huazhong Agricultural University,P.R.China 3.中国科学院烟台海岸带研究所
推荐引用方式 GB/T 7714	LI Liu,ZHENG Mengmeng,MA Xiaofang,et al. Molecular, serological and biological characterization of a novel Apple stem pitting virus strain from a local pear variety grown in China[J]. 农业科学学报:英文版,2019,18.0(011):2549-2560.
APA	LI Liu.,ZHENG Mengmeng.,MA Xiaofang.,LI Yuanjun.,LI Qingyu.,...&HONG Ni.(2019).Molecular, serological and biological characterization of a novel Apple stem pitting virus strain from a local pear variety grown in China.农业科学学报:英文版,18.0(011),2549-2560.
MLA	LI Liu,et al."Molecular, serological and biological characterization of a novel Apple stem pitting virus strain from a local pear variety grown in China".农业科学学报:英文版 18.0.011(2019):2549-2560.