Functional analyses of TRAF6 gene in Argopecten scallops
Zhang, Xiaotong1; Wang, Xia1; Chen, Jieyu2; Chen, Min3; Lu, Xia3; Ning, Junhao3; Liu, Haijun4; Liu, Guilong4; Xu, Xin4; Qu, Xiaoxu1; Yu, Kai1; Xu, He5,6; Wang, Chunde1,3; Liu, Bo1
KeywordArgopecten irradians (Air) Argopecten purpuratus (Apu) Tumor necrosis factor (TNF) TNF receptor-associated factor (TRAF) Subcellular localization RNA interference (RNAi)
Corresponding AuthorWang, Chunde(chundewang2007@163.com) ; Liu, Bo(liubomusic@126.com)
AbstractThe tumor necrosis factor (TNF) receptor-associated factor (TRAF) family has been reported to be involved in many immune pathways. In a previous study, we identified 5 TRAF genes, including TRAF2, 3, 4, 6, and 7, in the bay scallop (Argopecten irradians, Air) and the Peruvian scallop (Argopecten purpuratus, Apu). Since TRAF6 is a key molecular link in the TNF superfamily, we conducted a series of studies targeting the TRAF6 gene in the Air and Apu scallops as well as their hybrid progeny, Aip (Air 9 x Apu d) and Api (Apu 9 x Air d). Subcellular localization assay showed that the Air-, Aip-, and Api-TRAF6 were widely distributed in the cytoplasm of the human embryonic kidney cell line (HEK293T). Additionally, dual-luciferase reporter assay revealed that among TRAF3, TRAF4, and TRAF6, only the overexpression of TRAF6 significantly activated NF-kappa B activity in the HEK293T cells in a dose-dependent manner. These results suggest a crucial role of TRAF6 in the immune response in Argopecten scallops. To investigate the specific immune mechanism of TRAF6 in Argopecten scallops, we conducted TRAF6 knockdown using RNA interference. Transcriptomic analyses of the TRAF6 RNAi and control groups identified 1194, 2403, and 1099 differentially expressed genes (DEGs) in the Air, Aip, and Api scallops, respectively. KEGG enrichment analyses revealed that these DEGs were primarily enriched in transport and catabolism, amino acid metabolism, peroxisome, lysosome, and phagosome pathways. Expression profiles of 28 key DEGs were confirmed by qRT-PCR assays. The results of this study may provide insights into the immune mechanisms of TRAF in Argopecten scallops and ultimately benefit scallop breeding.
Funding OrganizationNational Natural Science Foundation of China ; Earmarked Fund for Agriculture Seed Improvement Project of Shandong Province ; Scientific and Technological Project of Yantai, Shandong Province ; Earmarked Fund for Shandong Modern Agro-Industry Technology Research System ; Science and Technology Innovation Capacity Improvement Project of Shandong Province ; Natural Science Foundation of Shandong Province
Indexed BySCI
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:001188218600001
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Document Type期刊论文
Corresponding AuthorWang, Chunde; Liu, Bo
Affiliation1.Qingdao Agr Univ, Coll Marine Sci & Engn, Qingdao 266109, Shandong, Peoples R China
2.Qingdao 9 High Sch, Qingdao 266426, Shandong, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresour, Yantai 264003, Shandong, Peoples R China
4.Yantai Spring Sea AquaSeed Co Ltd, Yantai 264006, Peoples R China
5.Jiangsu Baoyuan Biotechnol Co Ltd, Lianyungang 222144, Peoples R China
6.Jiangsu Haitai MariTech Co Ltd, Lianyungang 222144, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Xiaotong,Wang, Xia,Chen, Jieyu,et al. Functional analyses of TRAF6 gene in Argopecten scallops[J]. FISH & SHELLFISH IMMUNOLOGY,2024,147:9.
APA Zhang, Xiaotong.,Wang, Xia.,Chen, Jieyu.,Chen, Min.,Lu, Xia.,...&Liu, Bo.(2024).Functional analyses of TRAF6 gene in Argopecten scallops.FISH & SHELLFISH IMMUNOLOGY,147,9.
MLA Zhang, Xiaotong,et al."Functional analyses of TRAF6 gene in Argopecten scallops".FISH & SHELLFISH IMMUNOLOGY 147(2024):9.
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