A Rapid Method for Detecting Microplastics Based on Fluorescence Lifetime Imaging Technology (FLIM)

doi:10.3390/toxics10030118

YIC-IR

	A Rapid Method for Detecting Microplastics Based on Fluorescence Lifetime Imaging Technology (FLIM)
	Zhou, Fang; Wang, Xin; Wang, Guangxin; Zuo, Yanxia
发表期刊	TOXICS
	2022-03-01
卷号	10 期号:3 页码:18
关键词	microplastics fluorescence lifetime phasor analysis fingerprint library
DOI	10.3390/toxics10030118
通讯作者	Zuo, Yanxia(yxzuo@ihb.ac.cn)
英文摘要	With the increasing use and release of plastic products, microplastics have rapidly accumulated in ecological environments. When microplastics enter the food chain, they cause serious harm to organisms and humans. Microplastics pollution has become a growing concern worldwide; however, there is still no standardized method for rapidly and accurately detecting microplastics. In this work, we used fluorescence lifetime imaging technology to detect four kinds of Nile red-stained and unstained microplastics, and the unique phasor fingerprints of different microplastics were obtained by phasor analysis. Tracing the corresponding pixels of the "fingerprint" in the fluorescence lifetime image allowed for the quick and intuitive identification of different microplastics and their location distributions in a mixed sample. In our work, compared with staining the four microplastics with a fluorescent dye, using the phasor "fingerprint library" formed by the autofluorescence lifetimes of the microplastics was more easily distinguished than microplastics in the mixed samples. The feasibility of this method was further tested by adding three single substances-SiO2, chitin and decabromodiphenyl ethane (DBDPE), and surface sediments to simulate interferent in the environment, and the results providing potential applications for the identification and analysis of microplastics in complex environments.
资助机构	Technical Innovation Project of Instrument and Equipment Function Development of the Chinese Academy of Sciences
收录类别	SCI
语种	英语
关键词[WOS]	NILE RED ; SIZED MICROPLASTICS ; PHASOR ANALYSIS ; MU-M ; MICRO ; QUANTIFICATION ; IDENTIFICATION ; MARINE ; IMPACT ; WATER
研究领域[WOS]	Environmental Sciences & Ecology ; Toxicology
WOS记录号	WOS:000776390700001
引用统计	被引频次：17[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/34805
专题	中国科学院烟台海岸带研究所
通讯作者	Zuo, Yanxia
作者单位	Chinese Acad Sci, Anal & Testing Ctr, Inst Hydrobiol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Fang,Wang, Xin,Wang, Guangxin,et al. A Rapid Method for Detecting Microplastics Based on Fluorescence Lifetime Imaging Technology (FLIM)[J]. TOXICS,2022,10(3):18.
APA	Zhou, Fang,Wang, Xin,Wang, Guangxin,&Zuo, Yanxia.(2022).A Rapid Method for Detecting Microplastics Based on Fluorescence Lifetime Imaging Technology (FLIM).TOXICS,10(3),18.
MLA	Zhou, Fang,et al."A Rapid Method for Detecting Microplastics Based on Fluorescence Lifetime Imaging Technology (FLIM)".TOXICS 10.3(2022):18.