Human inflammatory dendritic cells in malignant pleural effusions induce Th1 cell differentiation

doi:10.1007/s00262-020-02510-1

YIC-IR

	Human inflammatory dendritic cells in malignant pleural effusions induce Th1 cell differentiation
	Gu, Fei-fei 1; Wu, Jing-jing 1; Liu, Yang-yang 1; Hu, Yue 1; Liang, Jin-yan 1; Zhang, Kai 1; Li, Ming 2; Wang, Yan 3; Zhang, Yong-an 4; Liu, Li 1
发表期刊	CANCER IMMUNOLOGY IMMUNOTHERAPY
ISSN	0340-7004
	2020-02-12
页码	10
关键词	Inflammatory DC NSCLC Pleural effusion DC T cell
DOI	10.1007/s00262-020-02510-1
通讯作者	Liu, Li(liulist2013@163.com)
英文摘要	Dendritic cells are crucial for the initiation and regulation of immune responses against cancer and pathogens. DCs are heterogeneous and highly specialized antigen-presenting cells. Human DCs comprise several subsets with different phenotypes and functional properties. In the steady state, human DC subsets have been well studied. However, the components of DC subsets and their immune functions during the inflamed setting are poorly understood. We identified and characterized DC subsets in the malignant pleural effusions of NSCLC patients. We analyzed the capacity of these DC subsets to induce T-cell differentiation. We observed the presence of inflammatory DCs (infDCs) and macrophages in the malignant pleural effusions of NSCLC patients, as identified by the CD11C(+)HLA-DR(+)CD16(-)BDCA1(+) and CD11C(+)HLA-DR(+)CD16(+)BDCA1(-) phenotypes, respectively. InfDCs represented approximately 1% of the total light-density cells in the pleural effusion and were characterized by the expression of CD206, CD14, CD11b, and CD1 alpha, which were absent on blood DCs. InfDCs also expressed CD80, although at a low level. As infDCs did not express CD40, CD83 and CD275, they remained functionally immature. We found that TLR agonists promoted the maturation of infDCs. Compared with macrophages, infDCs had a weaker capacity to phagocytose necrotic tumor cell lysates. However, only infDCs induced autologous memory CD4(+) T-cell differentiation into Th1 cells. For the first time, we found that infDCs were present in the malignant pleural effusions of NSCLC patients. We conclude that infDCs represent a distinct human DC subset and induce Th1 cell differentiation in the presence of TLR agonists.
资助机构	National Key R&D Program of China ; National Natural Science Foundations of China
收录类别	SCI
语种	英语
关键词[WOS]	PERIPHERAL LYMPHOID ORGANS ; CANCER STATISTICS ; STEADY-STATE ; LUNG-CANCER ; IDENTIFICATION ; MICE ; IMMUNITY ; SUBSETS
研究领域[WOS]	Oncology ; Immunology
WOS记录号	WOS:000513043500002
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/34759
专题	中国科学院烟台海岸带研究所
通讯作者	Liu, Li
作者单位	1.Huazhong Univ Sci & Technol, Union Hosp, Tongji Med Coll, Canc Ctr, 1277 Jiefang Ave, Wuhan 430022, Peoples R China 2.Wuhan Pulm Hosp, Med Oncol, 28 Baofeng Rd, Wuhan 430030, Peoples R China 3.Chinese Acad Sci, Anal & Testing Ctr, Inst Hydrobiol, 7 East Lake South Rd, Wuhan 430072, Peoples R China 4.Huazhong Agr Univ, Coll Fisheries, 1 Shizishan St, Wuhan 430070, Peoples R China
推荐引用方式 GB/T 7714	Gu, Fei-fei,Wu, Jing-jing,Liu, Yang-yang,et al. Human inflammatory dendritic cells in malignant pleural effusions induce Th1 cell differentiation[J]. CANCER IMMUNOLOGY IMMUNOTHERAPY,2020:10.
APA	Gu, Fei-fei.,Wu, Jing-jing.,Liu, Yang-yang.,Hu, Yue.,Liang, Jin-yan.,...&Liu, Li.(2020).Human inflammatory dendritic cells in malignant pleural effusions induce Th1 cell differentiation.CANCER IMMUNOLOGY IMMUNOTHERAPY,10.
MLA	Gu, Fei-fei,et al."Human inflammatory dendritic cells in malignant pleural effusions induce Th1 cell differentiation".CANCER IMMUNOLOGY IMMUNOTHERAPY (2020):10.