The method relates to a method for preparing hyperoside and isoquercitrin simultaneously from dogbane leaves. The method includes subjecting the dogbane leaves which serve as a raw material to heating reflux or ultrasonic extraction, and obtaining an extract after decompressing concentration; adding water to the extract for dissolution, and removing pigment and fat soluble impurities through petroleum ether; performing aqueous phase dilution, preforming dynamic adsorption until saturation through at least three serially connected macroporous adsorption resin columns, feeding an eluting solution for elution, then performing elution through the eluting solution until no hyperoside and isoquercitrin flowing out, and collecting the effluent liquid; and performing compressing concentration on the effluent liquid, performing recrystallization in methanol, enabling the liquid to pass through a medium-pressure gradient eluting system filled with C18 reverse phase silica gel, and obtaining the hyperoside and the isoquercitrin simultaneously whose purities reach 90%-98%. According to the method, the sample throughput is large, the operation is simple, high-purity hyperoside and isoquercitrin are prepared simultaneously, and not only the cost is low, but also the method is suitable for large-scale preparation.
; 孔娜娜; 方圣涛
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