The invention relates to a method for extracting chlorogenic acid, and in particular relates to a method for refining chlorogenic acid from a honeysuckle crude extract. The method specifically comprises the following processes : a process of removing impurities by adopting a macroporous resin column A, a macroporous resin column B enrichment and purification process and a middle-pressure liquid chromatography (MPLC) refining process. The method comprises the following steps : by adopting a honeysuckle crude extract as a raw material, fully dissolving the raw material by using deionized water to prepare an aqueous solution of the honeysuckle crude extract, removing the impurities of the aqueous solution by virtue of macroporous resin column A adsorption, enabling an effluent liquid to directly pass through a macroporous resin column B, performing dynamic adsorption and saturation, feeding an impurity washing liquid to wash the impurities until the effluent liquid is colorless, eluting macroporous resin B by using an eluent until high performance liquid chromatography (HPLC) detects that no chlorogenic acid flows out, collecting the eluent, performing reduced pressure concentration on the eluent, and performing gradient elution by virtue of the MPLC provided with a C18 reverse phase silica gel column to prepare a chlorogenic acid product with the purity of more than 98%. According to the method disclosed by the invention, a process of preparing high-purity chlorogenic acid by using the MPLC is proposed for the first time; and the method is large in sample treatment quantity and simple to operate, and is suitable for large-scale preparation.
; 孙鹏程; 王建华
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