一种从金银花提取物中制备高纯度绿原酸的方法

	一种从金银花提取物中制备高纯度绿原酸的方法
其他名称	A Honeysuckle extract from preparing high purity chlorogenic acid method
专利类型	发明
专利号	2013103902135
	夏传海; 孙鹏程; 王建华; 孔娜娜
专利权人	中国科学院烟台海岸带研究所
申请日期	2013-08-30
	2016-03-23
专利状态	授权
授权国家	中国
摘要	本发明涉及一种从金银花提取物中制备高纯度绿原酸的方法，具体涉及膜分离和膜浓缩过程，大孔树脂吸附洗脱过程和聚酰胺精制过程。本发明以金银花提取物为原料，用去离子水溶解，经膜分离和膜浓缩后过大孔树脂柱，流加洗杂液洗杂，用洗脱液洗脱至高效液相色谱(HPLC)检测无绿原酸流出，并收集洗脱液，洗脱液经减压浓缩、聚酰胺柱吸附分离洗脱精制后，喷雾干燥，得绿原酸纯度为98%以上的高纯度绿原酸。该方法制备的绿原酸纯度极高，绿原酸损失少，操作简单，适合大规模生产。
其他摘要	The invention relates to a method for preparing a high-purity chlorogenic acid from a honeysuckle extract, and particularly relates to membrane separation and membrane concentration processes, a macroporous resin adsorption elution process and a polyamide refining process. The method comprises the following steps : with the honeysuckle extract as a raw material, dissolving the honeysuckle extract with deionized water; filtering through a macroporous resin column after membrane separation and membrane concentration; feeding an impurity washing liquid to wash impurities; eluting with an eluent until no chlorogenic acid flows out in high-performance liquid chromatography (HPLC) detection; collecting the eluent, concentrating the eluent at reduced pressure, and carrying out adsorption separation, elution and refining on a polyamide column; and carrying out spray drying, so as to obtain high-purity chlorogenic acid of which the purity of chlorogenic acid is over 98%. The chlorogenic acid prepared by the method is extremely high in purity, low in loss, simple to operate and suitable for large-scale production.
申请号	2013103902135
公开（公告）号	CN104418742B
IPC 分类号	C07C69/732 ; C07C67/56 ; C07C67/48
专利代理人	周秀梅 ; 李颖
代理机构	沈阳科苑专利商标代理有限公司 21002
CPC分类号	C07C67/48 ; C07C67/56
文献类型	专利
条目标识符	http://ir.yic.ac.cn/handle/133337/33982
专题	海岸带生物学与生物资源利用重点实验室
作者单位	中国科学院烟台海岸带研究所
推荐引用方式 GB/T 7714	夏传海,孙鹏程,王建华,等. 一种从金银花提取物中制备高纯度绿原酸的方法. 2013103902135[P]. 2016-03-23.