本发明属于工业微生物领域，具体涉及一种对乙醇耐受性显著提高的集胞藻PCC6803突变株及其应用。乙醇耐受相关基因为slr0599，其碱基序列为SEQ ID No.7所示。突变株为PCC 6803△slr0599已于2017年8月4日保存在于中国微生物菌种保藏管理委员会普通微生物中心(CGMCC)，地址北京市朝阳区北辰西路1号院3号，保藏编号CGMCC14333，分类学命名集胞藻Synechocystis sp.。本发明集胞藻突变株为耐受乙醇突变株，在1.5％(v/v)的乙醇胁迫下，该藻株的生长状态明显优于野生型藻株。本发明得到的乙醇耐受性藻株对利用集胞藻生产生物燃料乙醇具有重要的应用价值和借鉴意义，应用前景广泛。
其他摘要
Belonging to the field of industrial microorganisms, the invention in particular relates to a synechocystis PCC6803 mutant strain with significantly improved ethanol tolerance and application thereof. The ethanol tolerance related gene is slr0599, and the base sequence is shown as SEQ ID No.7. The mutant strain PCC 6803Deltaslr0599 is preserved on August 4, 2017 in China General Microbiological Culture Collection Center (CGMCC) located at No.3, 1st yard, Beichen west road, Chaoyang District, Beijing, the preservation number is CGMCC14333, and the strain is named as Synechocystis sp. by taxonomy. The synechocystis mutant strain is an ethanol tolerant mutant strain, and under 1.5% (v/v) ethanol stress, the growth state of the algal strain is obviously superior to that of wild algal strains. The ethanol tolerant algal strain obtained by the invention has important application value and referential significance for production of biofuel ethanol with synechocystis, and has wide application prospect.
; 王肖肖; 秦松
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