The invention relates to the field of algae inhibitors, the invention specifically relates to a sesquiterpene alcohol compound derived from algal epiphytic fungus, a preparation method thereof and anapplication of the sesquiterpene alcohol compound in the aspect of inhibiting microalgae. The specific structural formula is shown in the formula (I). The preparation method comprises the following steps : inoculating a fungal culture medium with Trichoderma virens Y13-3 for fermentation culture, and subjecting a fermentation product to separating and purifying, and the product is namely a sesquiterpene alcohol compound as shown in the formula (I). The median inhibition concentration of the sesquiterpene alcohol compound to the microalga can be up to 1.2 microgram/ml through the microalga activity inhibition experiment.
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