The invention relates to the field of algistat, and particularly relates to epoxy sesquiterpenoids sourcing from seaweed epiphytic fungus and a preparation method and application of the epoxy sesquiterpenoids in inhibiting microalgae. The specific structure is as shown in formula (I) as shown in the description, and the preparation method comprises the steps that inoculating seaweed epiphytic fungus Trichoderma virens Y13-3 into a fungus culture medium for fermenting culture, and separating and purifying a fermentation product, thus obtaining the epoxy sesquiterpenoids as shown in formula (I).For the epoxy sesquiterpenoids obtained according to the invention, according to a microalgae activity inhibition experiment, the median inhibitory concentration of the compound to microalgae can reach 0.33 mcg/ml.
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