The invention relates to the field of algistats, in particular to alga endophytic fungi derived cyclonerodiol sesquiterpene amide as well as a preparation method and application thereof to algal inhibition. The structure of the cyclonerodiol sesquiterpene amide is as shown in a formula (I) as shown in the specification. The preparation method comprises the steps of inoculating alga endophytic fungi trichoderma asperellum A-YMD-9-2 into a fungi medium for fermentation culture, and then performing separation and purification on a fermentation product to form the cyclonerodiol sesquiterpene amideas shown in the formula (I). The cyclonerodiol sesquiterpene amide has the advantage that microalgae activity inhibition experiments show that the median inhibitory concentration of a compound to microalgae can reach 6.9 microgrammes/ milliliter.
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