The invention relates to the field of alga inhibitors and in particular discloses a monocycle-cycle flores aurantii alkane type sesquiterpene derivative as well as a preparation method and applicationthereof in inhibiting algae. The monocycle-cycle flores aurantii alkane type sesquiterpene derivative has a structural formula (I) as shown in the specification. The preparation method comprises thefollowing steps : inoculating Trichoderma asperellum A-YMD-9-2 into a fungus culture medium, carrying out fermentation culture, and separating and purifying a fermentation product, thereby obtaining the monocycle-cycle flores aurantii alkane type sesquiterpene derivative of formula (I) as shown in the specification. Alga inhibition activity tests on the monocycle-cycle flores aurantii alkane type sesquiterpene derivative disclosed by the invention show that the compound has a median inhibitory concentration up to 2.6mg/ml upon microalgae.
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