The invention provides a cell-based hydrogen sulfide activity detection composite microfluidic chip device. The system comprises an upper layer plate and a lower layer plate, wherein the upper layer plate and the lower layer plate are fixedly embedded in the lower layer plate; a Christmas tree network structure is embedded in the upper layer plate; and concentration gradient is generated by utilizing laminar flow and mixed flow effects in a channel. In the device, the concentration forming layer is arranged on the upper layer plate, and the cell culture layer is arranged on the lower layer plate, so that the concentration forming layer and the cell culture layer are separated from each other, and cells are prevented from remaining in a channel outside a culture cavity and blocking a pipeline; and the concentration forming layer is arranged on the upper layer plate, and the cell culture layer is arranged on the lower layer plate. In the process of introducing cells, the cells are firstly added to the paper chip and then combined with the concentration gradient generating system after being completely attached to the paper substrate, and the number of the cells in each culture cavity is extremely similar, so that the reproducibility and the accuracy of analysis data are ensured.
; 陈令新
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