The invention relates to the technical field of microorganisms, in particular to fungi obtained from deep-sea cold spring sediments, obtained natural azosteroid derivatives, a preparation method of the natural azosteroid derivatives and application of the natural azosteroid derivatives in algae inhibition and bacteriostasis. The preparation method comprises the following steps of : inoculating Penicillium oxalicum 13-37 from deep sea cold spring sediment into a fungus culture medium for fermentation culture; and separating and purifying a fermentation product to obtain the azosteroid derivative shown in the formula (I). The azosteroid derivative obtained by the invention has anti-microalgae and antibacterial activities.
; 刘雅萍
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