The utility model discloses a zebra fish gradient dissolved oxygen experimental device and relates to the technical field of experimental devices. The zebra fish gradient dissolved oxygen experimental device, By arranging the micro-fluidic chip, the peristaltic pump and the like, embedding the internal concentration gradient former into the microfluidic chip, injecting liquids with different dissolved oxygen concentrations into the two injection ports, capable of simultaneously generating a plurality of dissolved oxygen concentrations having a gradient, can be conveniently used in different dissolved oxygen concentrations, observing and testing zebra fish cell, embryo and larva, and because of its small volume, it is convenient to observe the growth state of organism under the microscope, Simultaneously, through the through holes formed in the silica gel pad and the transparent characteristic of the micro-fluidic chip, the growth states of zebra fish cells, embryos and larvae in the micro-fluidic chip can be observed at any time through a microscope conveniently.
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