The present invention discloses a sample pretreatment device of a flow cytometer, which comprises a multi-channel pressure pump, a sheath fluid container, a sample fluid container and a pretreatment pipeline. The pretreatment pipeline consists of three sections of a symmetrical pipeline, a linear pipeline and a spiral pipeline, wherein the symmetrical pipeline, the linear pipeline and the spiral pipeline are connected in sequence; The middle position of the top of the symmetrical pipeline is connected with a sheath liquid inlet; The middle position of the bottom of the symmetrical pipeline is connected with the sample inlet and is communicated with the linear pipeline; The spiral pipeline is provided with three outlets which are an inner outlet, an outer outlet and a middle outlet respectively; The sheath liquid container is connected with the multi-channel pressure pump and the sheath liquid inlet through a pipe; The sample liquid container is connected to the multi-channel pressure pump and the sample inlet via a tube. The device is used for removing debris cells and adhesive cells in a cell sample, so that the stability and the uniformity of the cell sample are improved, and the accuracy of a result is improved.
; 刘萍; 王文海
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