Effects of glyphosate exposure on gut-liver axis: Metabolomic and mechanistic analysis in grass carp (Ctenopharyngodon idellus)

doi:10.1016/j.scitotenv.2023.166062

	Effects of glyphosate exposure on gut-liver axis: Metabolomic and mechanistic analysis in grass carp (Ctenopharyngodon idellus)
	Yan, Biao 1,2,3; Sun, Yumiao 1,2; Fu, Kaiyu 1,2; Zhang, Yindan 1,2; Lei, Lei 1,2; Men, Jun 4; Guo, Yongyong 1; Wu, Shengmin 5; Han, Jian 1,6; Zhou, Bingsheng 1
发表期刊	SCIENCE OF THE TOTAL ENVIRONMENT
ISSN	0048-9697
	2023-12-01
卷号	902 页码:12
关键词	Glyphosate Metabolomics Grass carp Gut-Liver axis
DOI	10.1016/j.scitotenv.2023.166062
通讯作者	Wu, Shengmin(wsm@nies.org) ; Han, Jian(hanjian@ihb.ac.cn)
英文摘要	Glyphosate, one of the most widely used herbicide worldwide, is potentially harmful to non-target aquatic organisms. However, the environmental health risks regarding impacts on metabolism homeostasis and underlying mechanisms remain unclear. Here we investigated bioaccumulation, metabolism disorders and mechanisms in grass carp after exposure to glyphosate. Higher accumulation of glyphosate and its major metabolite, aminomethylphosphonic acid, in the gut was detected. Intestinal inflammation, barrier damage and hepatic steatosis were caused by glyphosate exposure. Lipid metabolism disorder was confirmed by the decreased triglyceride, increased total cholesterol and lipoproteins in serum and decreased visceral fat. Metabolomics analysis found that glyphosate exposure significantly inhibited bile acids biosynthesis in liver with decreased total bile acids content, which was further supported by significant downregulations of cyp27a1, cyp8b1 and fxr. Moreover, the dysbiosis of gut microbiota contributed to the inflammation in liver and gut by increasing lipopolysaccharide, as well as to the declined bile acids circulation by reducing secondary bile acids. These results indicated that exposure to environmental levels of glyphosate generated higher bioaccumulation in gut, where evokedenterohepatic injury, intestinal microbiota dysbiosis and disturbed homeostasis of bile acids metabolism; then the functional dysregulation of the gut-liver axis possibly resulted in ultimate lipid metabolism disorder. These findings highlight the metabolism health risks of glyphosate exposure to fish in aquatic environment.
资助机构	Open Project of State Environmental Protection Key Laboratory of Pesticide Environmental Assessment and Pollution Control ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology
收录类别	SCI
语种	英语
关键词[WOS]	FARNESOID X RECEPTOR ; AQUATIC ORGANISMS ; BILE-ACIDS ; HERBICIDE ; MICROBIOTA ; ZEBRAFISH ; RESPONSES ; OBESITY ; HEALTH ; IMPACT
研究领域[WOS]	Environmental Sciences & Ecology
WOS记录号	WOS:001058033800001
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/33493
专题	海岸带生物学与生物资源利用重点实验室
通讯作者	Wu, Shengmin; Han, Jian
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Hubei Univ Sci & Technol, Xianning Med Coll, Xianning Engn Res Ctr Hlth Environm, Xianning 437100, Peoples R China 4.Chinese Acad Sci, Inst Hydrobiol, Anal & Testing Ctr, Wuhan 430072, Peoples R China 5.Minist Ecol & Environm, Nanjing Inst Environm Sci, Nanjing 210042, Peoples R China 6.Chinese Acad Sci, Inst Hydrobiol, Donghu South Rd, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Yan, Biao,Sun, Yumiao,Fu, Kaiyu,et al. Effects of glyphosate exposure on gut-liver axis: Metabolomic and mechanistic analysis in grass carp (Ctenopharyngodon idellus)[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2023,902:12.
APA	Yan, Biao.,Sun, Yumiao.,Fu, Kaiyu.,Zhang, Yindan.,Lei, Lei.,...&Zhou, Bingsheng.(2023).Effects of glyphosate exposure on gut-liver axis: Metabolomic and mechanistic analysis in grass carp (Ctenopharyngodon idellus).SCIENCE OF THE TOTAL ENVIRONMENT,902,12.
MLA	Yan, Biao,et al."Effects of glyphosate exposure on gut-liver axis: Metabolomic and mechanistic analysis in grass carp (Ctenopharyngodon idellus)".SCIENCE OF THE TOTAL ENVIRONMENT 902(2023):12.