K252a inhibition of sperm viability for efficient crossbreeding of hermaphroditic bivalves

doi:10.1016/j.aquaculture.2023.739930

	K252a inhibition of sperm viability for efficient crossbreeding of hermaphroditic bivalves
	Liu, Tian 1,2,3; Wei, Huilan 1; Zhang, Lijing 1; Liu, Liangjie 1; Li, Yajuan 1; Shu, Ya 1; Yang, Yaxin 1; Zhao, Baojun 1; Xing, Qiang 1; Zeng, Qifan 1,4; Wang, Chunde 5,6; Wang, Shi 1,2,3,4,7; Bao, Zhenmin 1,4,7; Zhang, Lingling 1,2,3
发表期刊	AQUACULTURE
ISSN	0044-8486
	2023-12-15
卷号	577 页码:9
关键词	K252a Sperm motility Crossbreeding Simultaneous hermaphrodite Argopecten irradians Argopecten purpuratus
DOI	10.1016/j.aquaculture.2023.739930
通讯作者	Zhang, Lingling(lingling80@ouc.edu.cn)
英文摘要	Bivalve mollusks are economically important shellfish, representing 56.3% of aquaculture production. Cross-breeding is a popular approach for the genetic improvement of aquaculture species, including bivalves. However, it is difficult for simultaneous hermaphrodites due to the difficulty in collecting unfertilized oocytes. Male infertility or subfertility could be a solution. Considering that protein phosphorylation is critical in spermato-genesis, we investigated the effects of the protein kinase inhibitor K252a on germ cell development, gamete quality and embryonic/larval development of the simultaneously hermaphroditic scallop Argopecten irradians and evaluated its feasibility in crossbreeding. The results showed that 20% K252a injection at the gonad growth stage led to significant decreases in sperm number and progressive motility rate. Abnormal axoneme arrange-ment in flagella may have caused reduced sperm motility. Normal oocytes fertilized with K252a-treated sperm showed significant decreases in fertilization rate (38.24%), cleavage rate (83.42%) and D-stage survival rate (15.93%), and K252a-treated oocytes did not show obvious defects in early development. Interspecific hybrid-ization using K252a-treated A. irradians fertilized with Argopecten purpuratus sperm resulted in a 3.19-fold in-crease in the hybrid ratio (67%) over that of the control group (16%). Our study indicated that K252a influenced sperm development without affecting oocytes in A. irradians, indicating that K252a treatment may facilitate the mass production of crossbreeds and hybrids for simultaneous hermaphrodites. The approach reported here will greatly assist in genetic improvement programs for mollusk hermaphrodites.
资助机构	National Key Research and Devel-opment Program of China ; Na-tional Natural Science Foundation of China ; Key R amp; D Project of Shandong Province
收录类别	SCI
语种	英语
关键词[WOS]	PROTEIN-KINASE-C ; MALE-INFERTILITY ; BAY SCALLOP ; MICE DEFICIENT ; GROWTH ; MOTILITY ; DIFFERENTIATION ; SURVIVAL ; FLAGELLA ; LARVAE
研究领域[WOS]	Fisheries ; Marine & Freshwater Biology
WOS记录号	WOS:001051039500001
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/33457
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
通讯作者	Zhang, Lingling
作者单位	1.Ocean Univ China, MOE Key Lab Marine Genet & Breeding, Qingdao, Peoples R China 2.Laoshan Lab, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Laoshan Lab, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China 4.Ocean Univ China, Sanya Oceanog Inst, Lab Trop Marine Germplasm Resources & Breeding Eng, Sanya, Peoples R China 5.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Ctr Ocean Mega Sci, Yantai, Peoples R China 6.Qingdao Agr Univ, Coll Marine Sci & Engn, Qingdao, Peoples R China 7.Southern Marine Sci & Engn Guangdong Lab Guangzhou, Guangzhou, Peoples R China
推荐引用方式 GB/T 7714	Liu, Tian,Wei, Huilan,Zhang, Lijing,et al. K252a inhibition of sperm viability for efficient crossbreeding of hermaphroditic bivalves[J]. AQUACULTURE,2023,577:9.
APA	Liu, Tian.,Wei, Huilan.,Zhang, Lijing.,Liu, Liangjie.,Li, Yajuan.,...&Zhang, Lingling.(2023).K252a inhibition of sperm viability for efficient crossbreeding of hermaphroditic bivalves.AQUACULTURE,577,9.
MLA	Liu, Tian,et al."K252a inhibition of sperm viability for efficient crossbreeding of hermaphroditic bivalves".AQUACULTURE 577(2023):9.