Application of environmental DNA metabarcoding and quantitative PCR to detect blooming jellyfish in a temperate bay of northern China

doi:10.1002/ece3.10669

	Application of environmental DNA metabarcoding and quantitative PCR to detect blooming jellyfish in a temperate bay of northern China
	Peng, Saijun 1,2; Wang, Lei1,2 ; Ma, Yuanqing 3; Ye, Lijing 1; Hou, Chaowei 1,2; Liu, Yongliang 1,2; Li, Yongxue 1,2; Sun, Tingting 1,2; Zhao, Jianmin1,2 ; Dong, Zhijun1,2,4
发表期刊	ECOLOGY AND EVOLUTION
ISSN	2045-7758
	2023-11-01
卷号	13 期号:11 页码:13
关键词	Aurelia coerulea environmental DNA metabarcoding jellyfish blooms quantitative PCR
DOI	10.1002/ece3.10669
通讯作者	Dong, Zhijun(zjdong@yic.ac.cn)
英文摘要	Frequently occurring jellyfish blooms have severe impacts on the socioeconomics of coastal areas, which stress the importance of early detection and assessments of blooming jellyfish taxa. Environmental DNA (eDNA) techniques (quantitative PCR and eDNA metabarcoding) have the advantage of high sensitivity and are an emerging powerful tool for investigations of target species. However, a comprehensive analysis of the biodiversity and biomass of jellyfish taxa in the target area by combining the two eDNA techniques is still lacking. Here, we developed eDNA metabarcoding and quantitative PCR for the detection and assessment of jellyfish taxa in the temperate Yantai Sishili Bay (YSB) and estimated the spatial distribution of Aurelia coerulea. Species-specific quantitative PCR assays targeting the mitochondrial cytochrome c oxidase subunit I gene of A. coerulea were developed. Additionally, eDNA metabarcoding based on the mitochondrial 16S rDNA sequences identified six jellyfish species in YSB. Moreover, our results indicate that A. coerulea aggregations were more likely to occur in the inner part of the bay than in the outer part, and they gathered in the bottom layer of seawater rather than in the surface layer. Our results demonstrate the potential of two eDNA techniques in jellyfish biomass investigation and jellyfish taxa detection. These eDNA techniques may contribute to the discovery of jellyfish aggregation so as to achieve early warning of large-scale jellyfish blooms in coastal areas.
资助机构	This work was supported by grants from the National Science amp; Technology Fundamental Resources Investigation Program of China (2022FY100603), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA23050301), the Key Project of N ; National Science amp; Technology Fundamental Resources Investigation Program of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Key Project of NSFC-Shandong Joint Fund ; National Natural Science Foundation of China ; Taishan Scholars Program
收录类别	SCI
语种	英语
关键词[WOS]	AURELIA-AURITA S.L. ; SEA ; SCYPHOZOA ; COMMUNITY ; CNIDARIA ; PRESSURE ; CHOICE ; LARVAE ; QPCR
研究领域[WOS]	Environmental Sciences & Ecology ; Evolutionary Biology
WOS记录号	WOS:001093328000001
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/33292
专题	海岸带生物学与生物资源利用重点实验室支撑部门_中国科学院牟平海岸带环境综合试验站海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
通讯作者	Dong, Zhijun
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Shandong Marine Resource & Environm Res Inst, Shandong Key Lab Marine Ecol Restorat, Yantai, Shandong, Peoples R China 4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Peng, Saijun,Wang, Lei,Ma, Yuanqing,et al. Application of environmental DNA metabarcoding and quantitative PCR to detect blooming jellyfish in a temperate bay of northern China[J]. ECOLOGY AND EVOLUTION,2023,13(11):13.
APA	Peng, Saijun.,Wang, Lei.,Ma, Yuanqing.,Ye, Lijing.,Hou, Chaowei.,...&Dong, Zhijun.(2023).Application of environmental DNA metabarcoding and quantitative PCR to detect blooming jellyfish in a temperate bay of northern China.ECOLOGY AND EVOLUTION,13(11),13.
MLA	Peng, Saijun,et al."Application of environmental DNA metabarcoding and quantitative PCR to detect blooming jellyfish in a temperate bay of northern China".ECOLOGY AND EVOLUTION 13.11(2023):13.