YIC-IR  > 海岸带生物学与生物资源利用重点实验室
Application of environmental DNA metabarcoding and quantitative PCR to detect blooming jellyfish in a temperate bay of northern China
Peng, Saijun1,2; Wang, Lei1,2; Ma, Yuanqing3; Ye, Lijing1; Hou, Chaowei1,2; Liu, Yongliang1,2; Li, Yongxue1,2; Sun, Tingting1,2; Zhao, Jianmin1,2; Dong, Zhijun1,2,4
Source PublicationECOLOGY AND EVOLUTION
ISSN2045-7758
2023-11-01
Volume13Issue:11Pages:13
KeywordAurelia coerulea environmental DNA metabarcoding jellyfish blooms quantitative PCR
DOI10.1002/ece3.10669
Corresponding AuthorDong, Zhijun(zjdong@yic.ac.cn)
AbstractFrequently occurring jellyfish blooms have severe impacts on the socioeconomics of coastal areas, which stress the importance of early detection and assessments of blooming jellyfish taxa. Environmental DNA (eDNA) techniques (quantitative PCR and eDNA metabarcoding) have the advantage of high sensitivity and are an emerging powerful tool for investigations of target species. However, a comprehensive analysis of the biodiversity and biomass of jellyfish taxa in the target area by combining the two eDNA techniques is still lacking. Here, we developed eDNA metabarcoding and quantitative PCR for the detection and assessment of jellyfish taxa in the temperate Yantai Sishili Bay (YSB) and estimated the spatial distribution of Aurelia coerulea. Species-specific quantitative PCR assays targeting the mitochondrial cytochrome c oxidase subunit I gene of A. coerulea were developed. Additionally, eDNA metabarcoding based on the mitochondrial 16S rDNA sequences identified six jellyfish species in YSB. Moreover, our results indicate that A. coerulea aggregations were more likely to occur in the inner part of the bay than in the outer part, and they gathered in the bottom layer of seawater rather than in the surface layer. Our results demonstrate the potential of two eDNA techniques in jellyfish biomass investigation and jellyfish taxa detection. These eDNA techniques may contribute to the discovery of jellyfish aggregation so as to achieve early warning of large-scale jellyfish blooms in coastal areas.
Funding OrganizationThis work was supported by grants from the National Science amp; Technology Fundamental Resources Investigation Program of China (2022FY100603), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA23050301), the Key Project of N ; National Science amp; Technology Fundamental Resources Investigation Program of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Key Project of NSFC-Shandong Joint Fund ; National Natural Science Foundation of China ; Taishan Scholars Program
Indexed BySCI
Language英语
WOS KeywordAURELIA-AURITA S.L. ; SEA ; SCYPHOZOA ; COMMUNITY ; CNIDARIA ; PRESSURE ; CHOICE ; LARVAE ; QPCR
WOS Research AreaEnvironmental Sciences & Ecology ; Evolutionary Biology
WOS IDWOS:001093328000001
Citation statistics
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/33292
Collection海岸带生物学与生物资源利用重点实验室
支撑部门_中国科学院牟平海岸带环境综合试验站
海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
Corresponding AuthorDong, Zhijun
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai, Shandong, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Shandong Marine Resource & Environm Res Inst, Shandong Key Lab Marine Ecol Restorat, Yantai, Shandong, Peoples R China
4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Shandong, Peoples R China
Recommended Citation
GB/T 7714
Peng, Saijun,Wang, Lei,Ma, Yuanqing,et al. Application of environmental DNA metabarcoding and quantitative PCR to detect blooming jellyfish in a temperate bay of northern China[J]. ECOLOGY AND EVOLUTION,2023,13(11):13.
APA Peng, Saijun.,Wang, Lei.,Ma, Yuanqing.,Ye, Lijing.,Hou, Chaowei.,...&Dong, Zhijun.(2023).Application of environmental DNA metabarcoding and quantitative PCR to detect blooming jellyfish in a temperate bay of northern China.ECOLOGY AND EVOLUTION,13(11),13.
MLA Peng, Saijun,et al."Application of environmental DNA metabarcoding and quantitative PCR to detect blooming jellyfish in a temperate bay of northern China".ECOLOGY AND EVOLUTION 13.11(2023):13.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Peng, Saijun]'s Articles
[Wang, Lei]'s Articles
[Ma, Yuanqing]'s Articles
Baidu academic
Similar articles in Baidu academic
[Peng, Saijun]'s Articles
[Wang, Lei]'s Articles
[Ma, Yuanqing]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Peng, Saijun]'s Articles
[Wang, Lei]'s Articles
[Ma, Yuanqing]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.