Whole-Genome Re-sequencing and Transcriptome Reveal Candidate Genes and Pathways Associated with Hybrid Sterility in Hermaphroditic Argopecten Scallops

doi:10.1007/s10126-023-10247

	Whole-Genome Re-sequencing and Transcriptome Reveal Candidate Genes and Pathways Associated with Hybrid Sterility in Hermaphroditic Argopecten Scallops
	Yu, Tieying 1,2; Ning, Junhao 1; Wang, Fukai 3; Liu, Guilong 4; Wang, Quanchao 1; Xu, Xin 4; Wang, Chunde 1,2,3; Lu, Xia1
发表期刊	MARINE BIOTECHNOLOGY
ISSN	1436-2228
	2023-08-26
页码	16
关键词	Hermaphroditic Argopecten scallops Hybrid sterility Gametogenesis Meiosis and mitosis Apoptosis
DOI	10.1007/s10126-023-10247
通讯作者	Lu, Xia(xialu@yic.ac.cn)
英文摘要	The interspecific hybrid scallops generated from the hermaphroditic bay scallops (Argopecten irradians) and Peruvian scal-lops (Argopecten purpuratus) showed significant heterosis in growth. However, its sterility limits large-scale hybridization and hinders the development of the scallop breeding industry. Hybrid sterility is regulated by plenty of genes and involves a range of biochemical and physiological transformations. In this study, whole-genome re-sequencing and transcriptomic analysis were performed in sterile and fertile hybrid scallops. The potential genetic variations and abnormally expressed genes were detected to explore the mechanism underlying hybrid sterility in hermaphroditic Argopecten scallops. Compared with fertile hybrids, 24 differentially expressed genes (DEGs) with 246 variations were identified to be related to fertility regulation, which were mainly enriched in germarium-derived egg chamber formation, spermatogenesis, spermatid develop-ment, mismatch repair, mitotic and meiotic cell cycles, Wnt signaling pathway, MAPK signaling pathway, calcium modu-lating pathway, and notch signaling pathway. Specifically, variation and abnormal expression of these genes might inhibit the progress of mitosis and meiosis, promote cell apoptosis, and impede the genesis and maturation of gametes in sterile hybrid scallops. Eleven DEGs (XIAP, KAZN, CDC42, MEIS1, SETD1B, NOTCH2, TRPV5, M-EXO1, GGT1, SBDS, and TBCEL) were confirmed by qRT-PCR validation. Our findings may enrich the determination mechanism of hybrid sterility and provide new insights into the use of interspecific hybrids for extensive breeding.
资助机构	National Natural Science Foundation of China ; Earmarked Fund for Agriculture Seed Improvement Project of Shandong Province ; Scientific and Technological Project of Yantai, Shandong Province ; Earmarked Fund for Shandong Modern AgroIndustry Technology Research System ; Science and Technology Innovation Capacity Improvement Project in Shandong Province
收录类别	SCI
语种	英语
关键词[WOS]	DNA MISMATCH REPAIR ; BAY SCALLOP ; OOCYTE
研究领域[WOS]	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS记录号	WOS:001063657300001
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/32969
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
通讯作者	Lu, Xia
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresour, Yantai 264003, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Qingdao Agr Univ, Coll Marine Sci & Engn, Qingdao 266109, Shandong, Peoples R China 4.Yantai Spring Sea AquaSeed Ltd, Yantai 264006, Peoples R China
推荐引用方式 GB/T 7714	Yu, Tieying,Ning, Junhao,Wang, Fukai,et al. Whole-Genome Re-sequencing and Transcriptome Reveal Candidate Genes and Pathways Associated with Hybrid Sterility in Hermaphroditic Argopecten Scallops[J]. MARINE BIOTECHNOLOGY,2023:16.
APA	Yu, Tieying.,Ning, Junhao.,Wang, Fukai.,Liu, Guilong.,Wang, Quanchao.,...&Lu, Xia.(2023).Whole-Genome Re-sequencing and Transcriptome Reveal Candidate Genes and Pathways Associated with Hybrid Sterility in Hermaphroditic Argopecten Scallops.MARINE BIOTECHNOLOGY,16.
MLA	Yu, Tieying,et al."Whole-Genome Re-sequencing and Transcriptome Reveal Candidate Genes and Pathways Associated with Hybrid Sterility in Hermaphroditic Argopecten Scallops".MARINE BIOTECHNOLOGY (2023):16.