Effects of acute and chronic thermal stress on survival, apoptosis, and transcriptional responses of <i>Scapharca broughtonii</i>

doi:10.1007/s00343-022-2092-3

	Effects of acute and chronic thermal stress on survival, apoptosis, and transcriptional responses of Scapharca broughtonii
	Zou, Desheng 1,2; Cao, Weian 1; Liu, Guilong 3; Ning, Junhao 1,2; Lu, Xia1,2 ; Wang, Jinjing 1,2; Chen, Min1,2 ; Liu, Bo 4; Zhang, Jinsheng 5; Wang, Chunde 1,3,4
发表期刊	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	2096-5508
	2023-08-26
页码	11
关键词	Scapharca broughtonii thermal stress apoptosis necrosis gene expression
DOI	10.1007/s00343-022-2092-3
通讯作者	Ning, Junhao(jhning@yic.ac.cn)
英文摘要	Ocean warming is altering the habitats of marine invertebrates, which has resulted in an increased physiological stress to marine molluscs, especially those intertidal bivalves, such as the ark shell Scapharca broughtonii. We investigated the physiological and transcriptional responses of ark shells to acute and chronic thermal stress results showed that at 33 degrees C, a significantly higher cumulative mortality (55.7%) occurred under acute thermal stress than chronic thermal stress. The apoptosis rate of hemocytes was sustained at higher levels and the necrosis rate was increased significantly in a time-dependent manner under acute thermal stress. However, under chronic thermal stress, the apoptosis and necrosis rates exhibited similar change trends: a rapid increase followed by a gradual decline and sustained at a relatively high level until the end of the experiment. The expressions of heat shock protein genes (HSP20 and HSP90), apoptosis-related genes (TRAF6, GRP78, NIX, and Casp-3), antioxidative-related genes (GST and MRP) and cellular detoxification-related genes (HbIIB, NOS -1, HO -1, and ENO -1) were up regulated significantly under both acute and chronic thermal stresses. These results demonstrated that the anti-apoptotic system, antioxidant defense system, cellular detoxification system, and heat shock proteins (HSPs) played vital roles for ark shells in response to thermal stress. As acute thermal stress can result in irreversible damage to marine molluscs, it is thus advised that chronic thermal stress should be used to select thermal-resistant ark shell strains.
资助机构	We are grateful to all the laboratory members and anonymous reviewers for their technical advice and helpful discussions.
收录类别	SCI
语种	英语
关键词[WOS]	NITRIC-OXIDE SYNTHASE ; HEAT-SHOCK PROTEINS ; OXIDATIVE STRESS ; TEMPERATURE ; EXPRESSION ; ADAPTATION
研究领域[WOS]	Marine & Freshwater Biology ; Oceanography
WOS记录号	WOS:001070923300005
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/32846
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
通讯作者	Ning, Junhao
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Yantai Spring Sea AquaSeed Co Ltd, Yantai 264006, Peoples R China 4.Qingdao Agr Univ, Qingdao 266109, Peoples R China 5.Zhaoyuan Xiadian Agr Technol Promot Stn, Yantai 265415, Peoples R China
推荐引用方式 GB/T 7714	Zou, Desheng,Cao, Weian,Liu, Guilong,et al. Effects of acute and chronic thermal stress on survival, apoptosis, and transcriptional responses of Scapharca broughtonii[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2023:11.
APA	Zou, Desheng.,Cao, Weian.,Liu, Guilong.,Ning, Junhao.,Lu, Xia.,...&Wang, Chunde.(2023).Effects of acute and chronic thermal stress on survival, apoptosis, and transcriptional responses of Scapharca broughtonii.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,11.
MLA	Zou, Desheng,et al."Effects of acute and chronic thermal stress on survival, apoptosis, and transcriptional responses of Scapharca broughtonii".JOURNAL OF OCEANOLOGY AND LIMNOLOGY (2023):11.