Characteristic and antibacterial effect of a histone H2A and its preliminary roles in extracellular traps in manila clam Ruditapes philippinarum
Lv, Xiaojing1,2,6; Jiao, Chunli3; Chen, Yuying5; Chen, Lizhu5; Li, Xuan5; Liu, Xiangquan5; Yang, Dinglong1,2,4; Zhao, Jianmin1,2,4
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
2022-12-01
卷号131页码:77-83
关键词Ruditapes philippinarum Histone Antimicrobial activity Extracellular traps
DOI10.1016/j.fsi.2022.09.068
通讯作者Yang, Dinglong(dlyang@yic.ac.cn)
英文摘要In the present study, a histone H2A (designed as RpH2A) was identified and characterized from clam Ruditapes philippinarum, and its open reading frame (ORF) was of 387 bp encoding a polypeptide of 128 amino acids. The deduced amino acid sequence of RpH2A shared high identities ranging from 57.1% to 96.1% with that of other identified H2A. The mRNA expression of RpH2A was up-regulated significantly after Vibrio anguillarum chal-lenge. The recombinant RpH2A protein (rRpH2A) displayed significantly binding affinity to lipopolysaccharide (LPS) and peptidoglycan (PGN) in vitro, and also exhibited antimicrobial properties against Escherichia coli. In addition, the antimicrobial RpH2A was shown to co-localize with extracellular traps (ETs) released from he-mocytes induced by E. coli, suggesting that RpH2A might contribute to eliminate invading bacteria in clam ETs. Altogether, our data revealed that RpH2A could function as antimicrobial peptides, which might play a crucial role in the immune responses of hemocytes ETs in clams.
资助机构Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; Youth Innovation Promotion Association, Chinese Academy of Sciences ; Natural Science Foundation of Shandong Province ; Yantai Science and Technology Development Project
收录类别SCI
语种英语
关键词[WOS]MOLECULAR CHARACTERIZATION ; DISEASE VIRUS ; EXPRESSION ; HEMOCYTES ; IMMUNITY ; PROTEIN ; ANTIBODY ; H1
研究领域[WOS]Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号WOS:000876937200001
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/31979
专题海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
支撑部门_中国科学院牟平海岸带环境综合试验站
通讯作者Yang, Dinglong
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresour, Yantai 264003, Peoples R China
3.Yantai Ctr Food & Drug Control, Yantai 264003, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China
5.Shandong Marine Resources & Environm Res Inst, Shandong Key Lab Marine Ecol Restorat, Yantai 264006, Peoples R China
6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Lv, Xiaojing,Jiao, Chunli,Chen, Yuying,et al. Characteristic and antibacterial effect of a histone H2A and its preliminary roles in extracellular traps in manila clam Ruditapes philippinarum[J]. FISH & SHELLFISH IMMUNOLOGY,2022,131:77-83.
APA Lv, Xiaojing.,Jiao, Chunli.,Chen, Yuying.,Chen, Lizhu.,Li, Xuan.,...&Zhao, Jianmin.(2022).Characteristic and antibacterial effect of a histone H2A and its preliminary roles in extracellular traps in manila clam Ruditapes philippinarum.FISH & SHELLFISH IMMUNOLOGY,131,77-83.
MLA Lv, Xiaojing,et al."Characteristic and antibacterial effect of a histone H2A and its preliminary roles in extracellular traps in manila clam Ruditapes philippinarum".FISH & SHELLFISH IMMUNOLOGY 131(2022):77-83.
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