Identification of a novel CYP4V gene in the polychaete Perinereis aibuhitensis: transcriptional comparison with a CYP4B gene exposed to PAHs

doi:10.1007/s11356-022-18992-8

	Identification of a novel CYP4V gene in the polychaete Perinereis aibuhitensis: transcriptional comparison with a CYP4B gene exposed to PAHs
	Zhao, Huan 1,2; Wang, Lili 3; Lei, Yan 2,3; Wang, Yinan 2,3; Yang, Dazuo 2; Zhou, Yibing 2; Yuan, Xiutang1,3
发表期刊	ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
ISSN	0944-1344
	2022-02-19
页码	12
关键词	Polychaete Perinereis aibuhitensis PAHs CYP Liaohe Estuary
DOI	10.1007/s11356-022-18992-8
通讯作者	Yuan, Xiutang(xtyuan@yic.ac.cn)
英文摘要	Polychaete worms can biotransform polycyclic aromatic hydrocarbons (PAHs) in environments, and the cytochrome P450 (CYP) enzyme plays an important role in this process. Herein, a novel cytochrome P450 gene was identified and characterized from the polychaete worm Perinereis aibuhitensis. The full-length cDNA, which is named CYP4V82, is 1709 bp encoding a protein of 509 amino acids and has high similarity to CYP4V. The expression levels of CYP4V82 and CYP4BB4 (a CYP gene identified from P. aibuhitensis in a previous study, Chen et al. Mar Pollut Bull 64:1782-1788, 2012) exposure to various concentrations of benzo[a]pyrene (B[a]P) (0.5, 2, 4, and 8 mu g/L) and same mass concentrations of fluoranthene (Flu, 3.2 mu g/L), phenanthrene (Phe, 2.9 mu g/L), B[a]P (4.0 mu g/L) were detected to identify the function of the CYP4 family in P. aibuhitensis. Compared with CYP4BB4, CYP4V82 mRNA was minimally expressed on day 7 but highly sensitive on day 14. Notably, the expression levels of CYP4V82 and CYP4BB4 were relatively different in short-term responses to PAHs with different benzene rings of the same concentration. The expression of CYP4V82 in the B[a]P group was the highest, while that of CYP4BB4 in the Phe group was relatively higher than the two other groups. These findings suggest that PAHs are associated with the induction of CYP4V82 and CYP4BB4 expressions in P. aibuhitensis, which may have different efficiencies in the detoxification of PAHs.
资助机构	National Marine Public Welfare Research Project ; National Natural Science Foundation of China ; National Key R&D program of China ; Young Marine Science Foundation of SOA
收录类别	SCI
语种	英语
关键词[WOS]	POLYCYCLIC AROMATIC-HYDROCARBONS ; SCALLOP CHLAMYS-FARRERI ; CYTOCHROME-P450 GENES ; MOLECULAR-CLONING ; OXIDATIVE STRESS ; MESSENGER-RNA ; NEREIS-VIRENS ; BOHAI SEA ; EXPRESSION ; P450
研究领域[WOS]	Environmental Sciences & Ecology
WOS记录号	WOS:000758313700017
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/31508
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
通讯作者	Yuan, Xiutang
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Dalian Ocean Univ, Dalian 116023, Peoples R China 3.Natl Marine Environm Monitoring Ctr, Minist Ecol & Environm, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Huan,Wang, Lili,Lei, Yan,et al. Identification of a novel CYP4V gene in the polychaete Perinereis aibuhitensis: transcriptional comparison with a CYP4B gene exposed to PAHs[J]. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,2022:12.
APA	Zhao, Huan.,Wang, Lili.,Lei, Yan.,Wang, Yinan.,Yang, Dazuo.,...&Yuan, Xiutang.(2022).Identification of a novel CYP4V gene in the polychaete Perinereis aibuhitensis: transcriptional comparison with a CYP4B gene exposed to PAHs.ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,12.
MLA	Zhao, Huan,et al."Identification of a novel CYP4V gene in the polychaete Perinereis aibuhitensis: transcriptional comparison with a CYP4B gene exposed to PAHs".ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2022):12.