High-quality reannotation of the king scallop genome reveals no 'gene-rich' feature and evolution of toxin resistance

doi:10.1016/j.csbj.2021.08.0382001-0370/

	High-quality reannotation of the king scallop genome reveals no 'gene-rich' feature and evolution of toxin resistance
	Zeng, Qifan 1,2; Liu, Jing 1; Wang, Chunde 3,4,6; Wang, Hao 1; Zhang, Lingling 1; Hu, Jingjie 1,5; Bao, Lisui 1; Wang, Shi 1,2,5
发表期刊	COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
ISSN	2001-0370
	2021
卷号	19 页码:4954-4960
关键词	King scallop Genome sequencing Gene annotation Neurotoxin tolerance
DOI	10.1016/j.csbj.2021.08.0382001-0370/
通讯作者	Bao, Lisui(baolisui@ouc.edu.cn) ; Wang, Shi(swang@ouc.edu.cn)
英文摘要	ABSTR A C T The king scallop, Pecten maximus is a well-known, commercially important scallop species and is featured with remarkable tolerance to potent phytotoxins such as domoic acid. A high-quality genome can shed light on its biology and innovative evolution of toxin resistance. A reference genome has recently been published for P. maximus, however, it is suspicious that over 67,700 genes are annotated in this genome, which is unexpectedly larger than its close relatives of pectinids. Herein, we provide an improved high-quality chromosome-level reference genome assembly and annotation for the king scallop P. maximus. A final set of 26,995 genes is annotated after carefully checking and curation of the predicted gene models, which significantly improves the accuracy of gene structure information. The large number of gene dupli-cates in the previous genome is mainly distorted by the fragmented annotation. Through integrated genomic, evolutionary and transcriptomic analyses, we reveal that the Phi subfamily of ionotropic gluta-mate receptors (iGluRs) are well preserved in molluscs, and P. maximus experienced the rapid expansion of the Phi class of iGluR (GluF) gene family. The GluF genes exhibit ubiquitously high expression and altered sequence characteristics for ligand selectivity, which may contribute to the remarkable tolerance to neurotoxins in P. maximus. Taken together, our study disapproves the previous claim of the 'gene-rich' genome of this species and provides a high-quality genome assembly for further understanding of its biology and evolution of toxin resistance. (c) 2021 The Author(s). Published by Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology. This is an open access article under the CC BY-NC-ND license (http://creative-commons.org/licenses/by-nc-nd/4.0/).
资助机构	National Natural Science Foundation of China ; Funda-mental Research Funds for the Central Universities ; Project of Sanya Yazhouwan Science and Technology City Manage-ment Foundation ; Taishan Scholar Project Fund of Shandong Province of China
收录类别	SCI
语种	英语
关键词[WOS]	PARALYTIC SHELLFISH TOXINS ; DOMOIC ACID ; PREDICTION ; ALIGNMENT ; ALGORITHM ; PROVIDES ; SYSTEM ; CORE ; TOOL
研究领域[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS记录号	WOS:000704386800004
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/30751
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
通讯作者	Bao, Lisui; Wang, Shi
作者单位	1.Ocean Univ China, Coll Marine Life Sci, MOE Key Lab Marine Genet & Breeding & Sars Fang C, Qingdao 266003, Peoples R China 2.Pilot Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Yantai 264003, Peoples R China 5.Ocean Univ China, Sanya Oceanog Inst, Lab Trop Marine Germplasm Resources & Breeding En, Sanya 572000, Peoples R China 6.Qingdao Agr Univ, Qingdao 266109, Peoples R China
推荐引用方式 GB/T 7714	Zeng, Qifan,Liu, Jing,Wang, Chunde,et al. High-quality reannotation of the king scallop genome reveals no 'gene-rich' feature and evolution of toxin resistance[J]. COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL,2021,19:4954-4960.
APA	Zeng, Qifan.,Liu, Jing.,Wang, Chunde.,Wang, Hao.,Zhang, Lingling.,...&Wang, Shi.(2021).High-quality reannotation of the king scallop genome reveals no 'gene-rich' feature and evolution of toxin resistance.COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL,19,4954-4960.
MLA	Zeng, Qifan,et al."High-quality reannotation of the king scallop genome reveals no 'gene-rich' feature and evolution of toxin resistance".COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL 19(2021):4954-4960.