| Antibacterial activity and mechanism of a type-I ubiquitin from the clam Ruditapes philippinarum | |
Han, Yijing2,3; Chen, Lizhu4; Yang, Dinglong1,2
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| 发表期刊 | JOURNAL OF INVERTEBRATE PATHOLOGY
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| ISSN | 0022-2011 |
| 2021-07-01 | |
| 卷号 | 183页码:6 |
| 关键词 | Ruditapes philippinarum Ubiquitin Antibacterial activity Bacteriostatic activity |
| DOI | 10.1016/j.jip.2021.107602 |
| 通讯作者 | Yang, Dinglong(dlyang@yic.ac.cn) |
| 英文摘要 | In the present study, a ubiquitin (designated as RpUbi) was identified and characterized from clam Ruditapes philippinarum. Phylogenetic analysis strongly suggested that RpUbi was a member of the ubiquitin family. In nonstimulated clams, RpUbi transcripts were constitutively expressed in all examined tissues, especially in the gills and hemocytes. After Vibrio anguillarum challenge, expression of RpUbi mRNA in hemocytes was significantly upregulated. Recombinant RpUbi (rRpUbi) showed high antibacterial activity against Gram-positive and Gramnegative bacteria. Notably, membrane integrity and electrochemical assay indicated that rRpUbi could invade the inner layer. Moreover, DNA migration could be inhibited by rRpUbi in a concentration-dependent manner. In general, our results suggested that RpUbi played an important role in host defense against invading bacteria, perhaps through a DNA-binding process. |
| 资助机构 | Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; Natural Science Foundation of Shandong Province ; Yantai Science and Technology Development Project ; Youth Innovation Promotion Association of CAS |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | BROWN RING DISEASE ; MANILA CLAM ; MOLECULAR CHARACTERIZATION ; CONCERTED EVOLUTION ; POLYUBIQUITIN GENE ; EXPRESSION ; PROTEIN ; IDENTIFICATION ; PURIFICATION ; MEMBRANES |
| 研究领域[WOS] | Zoology |
| WOS记录号 | WOS:000670119900003 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/29453 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心 |
| 通讯作者 | Yang, Dinglong |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Shandong Marine Resource & Environm Res Inst, Yantai 264006, Peoples R China |
| 推荐引用方式 GB/T 7714 | Han, Yijing,Chen, Lizhu,Yang, Dinglong. Antibacterial activity and mechanism of a type-I ubiquitin from the clam Ruditapes philippinarum[J]. JOURNAL OF INVERTEBRATE PATHOLOGY,2021,183:6. |
| APA | Han, Yijing,Chen, Lizhu,&Yang, Dinglong.(2021).Antibacterial activity and mechanism of a type-I ubiquitin from the clam Ruditapes philippinarum.JOURNAL OF INVERTEBRATE PATHOLOGY,183,6. |
| MLA | Han, Yijing,et al."Antibacterial activity and mechanism of a type-I ubiquitin from the clam Ruditapes philippinarum".JOURNAL OF INVERTEBRATE PATHOLOGY 183(2021):6. |
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| Antibacterial activi（1158KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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