| Molecular characterization, expression and immune functions of two C-type lectin from Venerupis philippinarum | |
Xin, Zifan1; Yu, Daode6; Yang, Bowen1; Chen, Lizhu4; Hayouka, Zvi5; Chen, Xingfu1; Gong, Yitong1; Dai, Haiyu1; Wang, Lei1 ; Zhao, Yancui1; Liu, Xiaoli1; Yang, Dinglong2,3
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| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 2020-12-01 | |
| 卷号 | 107页码:260-268 |
| 关键词 | Venerupis philippinarum C-type lectin Molecular characterization Immune recognition |
| DOI | 10.1016/j.fsi.2020.10.006 |
| 通讯作者 | Liu, Xiaoli(lxlshz2006@163.com) ; Yang, Dinglong(dlyang@yic.ac.cn) |
| 英文摘要 | In the present study, two C-type lectins (designated as VpClec-3 and VpClec-4) were identified and characterized from the manila clam Venerupis philippinarum. Multiple alignment and phylogenetic relationship analysis strongly suggested that VpClec-3 and VpClec-4 belong to the C-type lectin family. In nonstimulated clams, the VpClec-3 transcript was dominantly expressed in the hepatopancreas, while the VpClec-4 transcript was mainly expressed in gill tissues. Both VpClec-3 and VpClec-4 mRNA expression was significantly upregulated following Vibrio anguillarum challenge. Recombinant VpClec-4 (rVpClec-4) was shown to bind lipopolysaccharide (LPS) and glucan in vitro, whereas recombinant VpClec-3 (rVpClec-3) only bound to glucan. In addition, rVpClec-3 and rVpClec-4 displayed broad agglutination activities towards Vibrio harveyi, Vibrio splendidus and V. anguillarum, while no agglutination activities towards Enterobacter cloacae or Aeromonas hydrophila were observed in rVpClec-3. Moreover, hemocyte phagocytosis was significantly enhanced by rVpClec-3 and rVpClec-4. All the results showed that VpClecs function as pattern recognition receptors (PRRs) with distinct recognition spectra and are potentially involved in the innate immune responses of V. philippinarum. |
| 资助机构 | Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; Natural Science Foundation of Shandong Province ; Yantai Science and Technology Development Project ; Marine and Fisheries Science and Technology Innovation Program of Shandong |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | RECOGNITION ; CLONING ; GENE ; HEMOCYTES ; IDENTIFICATION ; DISEASE ; CLAM |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000599882200029 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/27553 |
| 专题 | 支撑部门_中国科学院牟平海岸带环境综合试验站 中国科学院烟台海岸带研究所 海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心 |
| 通讯作者 | Liu, Xiaoli; Yang, Dinglong |
| 作者单位 | 1.Ludong Univ, Key Lab Marine Biotechnol Univ Shandong, Sch Life Sci, Yantai 264025, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China 4.Shandong Marine Resource & Environm Res Inst, Yantai 264006, Peoples R China 5.Hebrew Univ Jerusalem, Robert H Smith Fac Agr Food & Environm, Inst Biochem Food Sci & Nutr, IL-76100 Rehovot, Israel 6.Marine Biol Inst Shandong Prov, Qingdao 266002, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xin, Zifan,Yu, Daode,Yang, Bowen,et al. Molecular characterization, expression and immune functions of two C-type lectin from Venerupis philippinarum[J]. FISH & SHELLFISH IMMUNOLOGY,2020,107:260-268. |
| APA | Xin, Zifan.,Yu, Daode.,Yang, Bowen.,Chen, Lizhu.,Hayouka, Zvi.,...&Yang, Dinglong.(2020).Molecular characterization, expression and immune functions of two C-type lectin from Venerupis philippinarum.FISH & SHELLFISH IMMUNOLOGY,107,260-268. |
| MLA | Xin, Zifan,et al."Molecular characterization, expression and immune functions of two C-type lectin from Venerupis philippinarum".FISH & SHELLFISH IMMUNOLOGY 107(2020):260-268. |
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