Astaxanthin accumulation in Haematococcus pluvialis observed through Fourier-transform infrared microspectroscopy imaging

doi:10.1016/j.molstruc.2019.01.026

	Astaxanthin accumulation in Haematococcus pluvialis observed through Fourier-transform infrared microspectroscopy imaging
	Liu, JH ; Chen, XY ; Yang, XQ ; Chen, J ; Qi, ZM
发表期刊	JOURNAL OF MOLECULAR STRUCTURE
ISSN	0022-2860
	2019-04-15
卷号	1182 页码:119-122
关键词	Astaxanthin Carotenogenesis FTIR microspectroscopy imaging Haematococcus pluvialis Inhibitors
研究领域	Chemistry, Physical
DOI	10.1016/j.molstruc.2019.01.026
产权排序	[Liu, Jinghua; Chen, Xinyi] Anhui Sci & Technol Univ, Coll Life & Hlth Sci, Fengyang 233100, Peoples R China; [Yang, Xianqing] Minist Agr Peoples Republ China, Key Lab Aquat Prod Proc, Guangzhou 510300, Guangdong, Peoples R China; [Chen, Jun] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Qi, Zeming] Univ Sci & Technol China, Natl Synchrotron Radiat Lab, Hefei 230029, Anhui, Peoples R China
作者部门	中科院烟台海岸带研究所知识产出
英文摘要	Haematococcus pluvialis has emerged as a promising microalga for its rapid accumulation of astaxanthin. However, the underlying mechanism for astaxanthin biosynthesis is still unclear and needs further exploring in terms of the chemical changes of algal cell during the carotenogenesis pathway. In this study, the chemical changes were monitored in the algal cells exposed to carotenogenesis inhibitors by Fourier-transform infrared (FTIR) microspectroscopy imaging. The significant increase of signals at 3010 and 2925 cm(-1) suggests the accumulation of carotenoids during the algal cell encysting. In contrast, a relatively low level of carotenoids in the algal cells contacted to nicotine and diphenylamine were observed for the biosynthesis blocking of beta-carotene and astaxanthin. The decrease of the absorbance at 1740 and 1650 cm(-1) indicates that lipids, proteins and astaxanthin biosynthesis were also inhibited by nicotine and diphenylamine while the cell wall biosynthesis was not disturbed by the inhibitors for the strong absorbance band at 1035 cm(-1) assigned to polysaccharides. This work demonstrates that FTIR microspectroscopy imaging is an effective and none-invasive tool to analyze chemical changes and the components location in the single algal cell with high spatial resolution and it facilitates the study of carotenoid biosynthetic pathway. (C) 2019 Elsevier B.V. All rights reserved.
文章类型	Article
资助机构	Fund of Key Laboratory of Aquatic Product Processing, Ministry of Agriculture, China [NYJG201802] ; Research Projects of Anhui Science and Technology University for Key Discipline [AKZDXK2015B02]
收录类别	SCI
语种	英语
关键词[WOS]	GREEN-ALGA ; CULTIVATION ; LIGHT ; IR
研究领域[WOS]	Chemistry, Physical
WOS记录号	WOS:000458618500015
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/24958
专题	中国科学院烟台海岸带研究所知识产出
作者单位	1.Anhui Sci & Technol Univ, Coll Life & Hlth Sci, Fengyang 233100, Peoples R China; 2.Minist Agr Peoples Republ China, Key Lab Aquat Prod Proc, Guangzhou 510300, Guangdong, Peoples R China; 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; 4.Univ Sci & Technol China, Natl Synchrotron Radiat Lab, Hefei 230029, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Liu, JH,Chen, XY,Yang, XQ,et al. Astaxanthin accumulation in Haematococcus pluvialis observed through Fourier-transform infrared microspectroscopy imaging[J]. JOURNAL OF MOLECULAR STRUCTURE,2019,1182:119-122.
APA	Liu, JH,Chen, XY,Yang, XQ,Chen, J,&Qi, ZM.(2019).Astaxanthin accumulation in Haematococcus pluvialis observed through Fourier-transform infrared microspectroscopy imaging.JOURNAL OF MOLECULAR STRUCTURE,1182,119-122.
MLA	Liu, JH,et al."Astaxanthin accumulation in Haematococcus pluvialis observed through Fourier-transform infrared microspectroscopy imaging".JOURNAL OF MOLECULAR STRUCTURE 1182(2019):119-122.

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