| Immune recognition, antimicrobial and opsonic activities mediated by a sialic acid binding lectin from Ruditapes philippinarum | |
Li, SQ; Ruan, ZL; Yang, XY; Li, MZ; Yang, DL
| |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
 |
| ISSN | 1050-4648 |
| 2019-10 | |
| 卷号 | 93页码:66-72 |
| 关键词 | Ruditapes philippinarum Sialic acid-binding lectin Pattern recognition receptor Immune recognition |
| 研究领域 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| DOI | 10.1016/j.fsi.2019.07.027 |
| 产权排序 | [Li, Shengqiang; Ruan, Zeli; Yang, Xiyun; Li, Mingzhu] Ludong Univ, Coll Agr, Yantai, Peoples R China; [Yang, Dinglong] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China; [Yang, Dinglong] Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China |
| 通讯作者 | Li, Mingzhu(ldulimingzhu@163.com) ; Yang, Dinglong(dlyang@yic.ac.cn) |
| 作者部门 | 中国科学院牟平海岸带环境综合试验站 |
| 英文摘要 | In the present study, a sialic acid-binding lectin was identified and characterized from Manila clam Ruditapes philippinarum (designed as RpSABL-1). Multiple alignments strongly suggested that RpSABL-1 was a new member of the sialic acid-binding lectin family. In non-stimulated clams, RpSABL-1 transcripts were constitutively expressed in all five tested tissues, especially in hepatopancreas. After Vibrio anguillarum challenge, the expression of RpSABL-1 mRNA was significantly up-regulated at 6 h (P < 0.05), 12 h (P < 0.01) and 24 h (P < 0.01). Recombinant RpSABL-1 protein (rRpSABL-1) displayed apparent binding activities towards lipopolysaccharides (LPS) and peptidoglycan (PGN), but not to glucan or chitin in vitro. Coinciding with the PAMPs binding assay, rRpSABL-1 exhibited obvious agglutination activities against Gram-positive bacterium Staphyloccocus aureus, Gram-negative bacteria Escherichia coli, V. anguillarum and Vibrio harveyi. Meanwhile, rRpSABL-1 showed antibacterial activities against E. coli, and biofilm formation of E. coli could also be inhibited after incubated with rRpSABL-1. Moreover, the encapsulation, phagocytosis and chemotactic ability of hemocytes could be enhanced by rRpSABL-1. All these results suggested that RpSABL-1 could function as a pattern recognition receptor with versatile functions in the innate immune responses of R. philippinarum. |
| 文章类型 | Article |
| 资助机构 | Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDA23050303] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41806196] ; Key Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [KFJ-STS-ZDTP-023] ; Natural Science Foundation of Shandong ProvinceNatural Science Foundation of Shandong Province [ZR2019BD022] ; Youth Innovation Promotion Association CAS [2016196] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | C-TYPE LECTIN ; ANTIBACTERIAL ACTIVITY ; C1Q-DOMAIN-CONTAINING PROTEIN ; CHLAMYS-FARRERI ; EXPRESSION ; PEPTIDE ; MECHANISM ; MONOCYTES ; DEFENSIN ; DISEASE |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000488998300008 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/24845 |
| 专题 | 支撑部门_中国科学院牟平海岸带环境综合试验站 海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心 |
| 作者单位 | 1.Ludong Univ, Coll Agr, Yantai, Peoples R China; 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China; 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, SQ,Ruan, ZL,Yang, XY,et al. Immune recognition, antimicrobial and opsonic activities mediated by a sialic acid binding lectin from Ruditapes philippinarum[J]. FISH & SHELLFISH IMMUNOLOGY,2019,93:66-72. |
| APA | Li, SQ,Ruan, ZL,Yang, XY,Li, MZ,&Yang, DL.(2019).Immune recognition, antimicrobial and opsonic activities mediated by a sialic acid binding lectin from Ruditapes philippinarum.FISH & SHELLFISH IMMUNOLOGY,93,66-72. |
| MLA | Li, SQ,et al."Immune recognition, antimicrobial and opsonic activities mediated by a sialic acid binding lectin from Ruditapes philippinarum".FISH & SHELLFISH IMMUNOLOGY 93(2019):66-72. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Immune recognition, （2368KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论