Two c-type lectins from Venerupis philippinarum: Possible roles in immune recognition and opsonization

doi:10.1016/j.fsi.2019.09.009

	Two c-type lectins from Venerupis philippinarum: Possible roles in immune recognition and opsonization
	Zhang, JN ; Zhang, YF ; Chen, LZ ; Yang, JM ; Wei, QY ; Yang, BW ; Liu, XL ; Yang, DL
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
	2019-11
卷号	94 页码:230-238
关键词	Venerupis philippinarum c-type lectin Pattern recognition receptor Immune recognition
研究领域	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI	10.1016/j.fsi.2019.09.009
产权排序	[Zhang, Jianning; Zhang, Yifei; Yang, Jianmin; Yang, Bowen; Liu, Xiaoli] Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China; [Wei, Qianyu; Yang, Dinglong] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Moping Coastal Environm Res Stn, Yantai 264003, Peoples R China; [Yang, Dinglong] Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China; [Chen, Lizhu] Shandong Marine Resource & Environm Res Inst, Yantai 264006, Peoples R China
通讯作者	Liu, Xiaoli(lxlshz2006@163.com) ; Yang, Dinglong(dlyang@yic.ac.cn)
作者部门	中国科学院牟平海岸带环境综合试验站
英文摘要	In the study, two c type lectins were identified and characterized from the manila clam Venerupis philippinarum (designed as VpClec-1 and VpClec-2, respectively). Multiple alignments and phylogenetic analysis strongly suggested that they were new members of the c-type lectin superfamily. In normal tissue of clams, both VpClec-1 and VpClec-2 transcripts were highly expressed in the tissue of hepatopancreas. After Vibrio anguillartun challenge, the temporal expression of both VpClec-1 and VpClec-2 transcripts was up-regulated in the hemocytes of manila clams. The recombinant protein VpClec-1 (rVpClec-1) showed obvious binding activities to lipopolysaccharide (LPS), peptidoglycan (PGN), glucan and zymosan in vitro, while the recombinant protein VpClec-2 (rVpClec-2) could only bind LPS, glucan and zymosan. Coinciding with the PAMPs binding assay, both rVpClec-1 and rVpClec-2 displayed broad agglutination and antibacterial activities towards Vibrio harveyi, Vibrio splendidus, Vibrio arquillarum, Enterobacter cloacae and Aeromonas hydrophila. Moreover, the phagocytosis and encapsulation ability of hemocytes could be significantly enhanced by rVpClec-1 and rVpClec-2. Notably, the rVpClec-1 but not rVpClec-2 elicited a chemotactic response from hemocytes. All the results showed that VpClec-1 and VpClec-2 functioned as pattern recognition receptors (PRRs) with distinct recognition spectrum, and involved in the innate immune responses of manila clams.
文章类型	Article
资助机构	Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDA23050303] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41806196, 41506190] ; Natural Science Foundation of Shandong ProvinceNatural Science Foundation of Shandong Province [ZR2019BD022] ; 13th Five-year Informatization Plan of Chinese Academy of Sciences [XXH13506]
收录类别	SCI
语种	英语
关键词[WOS]	ACID-BINDING LECTIN ; MOLECULAR-CLONING ; LITOPENAEUS-VANNAMEI ; MANDUCA-SEXTA ; PROTEIN ; GENE ; DOMAIN ; PEPTIDOGLYCAN ; IMMULECTIN-2 ; ACTIVATION
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000496892400025
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/24822
专题	支撑部门_中国科学院牟平海岸带环境综合试验站海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
作者单位	1.Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China; 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Moping Coastal Environm Res Stn, Yantai 264003, Peoples R China; 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China; 4.Shandong Marine Resource & Environm Res Inst, Yantai 264006, Peoples R China
推荐引用方式 GB/T 7714	Zhang, JN,Zhang, YF,Chen, LZ,et al. Two c-type lectins from Venerupis philippinarum: Possible roles in immune recognition and opsonization[J]. FISH & SHELLFISH IMMUNOLOGY,2019,94:230-238.
APA	Zhang, JN.,Zhang, YF.,Chen, LZ.,Yang, JM.,Wei, QY.,...&Yang, DL.(2019).Two c-type lectins from Venerupis philippinarum: Possible roles in immune recognition and opsonization.FISH & SHELLFISH IMMUNOLOGY,94,230-238.
MLA	Zhang, JN,et al."Two c-type lectins from Venerupis philippinarum: Possible roles in immune recognition and opsonization".FISH & SHELLFISH IMMUNOLOGY 94(2019):230-238.

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