Molecular diversity and evolution of defensins in the manila clam Ruditapes philippinarum

doi:10.1016/j.fsi.2015.09.008

	Molecular diversity and evolution of defensins in the manila clam Ruditapes philippinarum
	Wang, Q; Zhang, LB; Yang, DL; Yu, Q; Li, F; Cong, M; Ji, CL; Wu, HF; Zhao, JM; Zhao, JM (reprint author), Chinese Acad Sci, Key Lab Coastal Environm Processes & Ecol Remedia, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China. jmzhao@yic.ac.cn
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
	2015-11-01
卷号	47 期号:1 页码:302-312
关键词	Defensin Manila Clam Diversity Positive Selection
DOI	10.1016/j.fsi.2015.09.008
产权排序	[Wang, Qing; Yang, Dinglong; Yu, Qian; Li, Fei; Cong, Ming; Ji, Chenglong; Wu, Huifeng; Zhao, Jianmin] Chinese Acad Sci, Key Lab Coastal Environm Processes & Ecol Remedia, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Zhang, Linbao] Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China; [Yang, Dinglong; Yu, Qian] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
作者部门	中科院海岸带环境过程与生态修复重点实验室
英文摘要	Four types of defensins were identified in Manila clam and designated as Rpdef1, Rpdef2, Rpdef3 and Rpdef4, which encoded a polypeptide of 49, 46, 45 and 42 amino acids, respectively. Sequence alignments indicated that Rpdef1 shared 46.9% identity with Rpdef2, 40.8% with Rpdef3, and 34.7% with Rpdef4. Analysis of transcript polymorphism showed that Rpdef3 accounted for about 60% frequency of Rpdefs occurrence in clams from three geographic origins (Dalian, Qingdao and Hangzhou). By quantitative real-time RT-PCR (qRT-PCR) analysis, the transcripts of Rpdefs were mainly detected in hemocytes and they responded sensitively to bacterial challenge in hemocytes. Evolutionary analysis indicated that all Rpdefs were under positive selection with positively selected basic amino acid residues detected in the C-terminal regions, which perhaps have a functional relevance by modifying the charge distribution of Rpdefs. The results also showed some lineages with dN/dS > 1, suggesting positive selection pressures existed in some lineages of phylogeny tree constructed by mollusk defensins. Overall, our results suggest that Rpdefs perhaps played important roles in host defense and positive selection is the major driving force in generating high diversity of defensins in the Manila clam. (C) 2015 Elsevier Ltd. All rights reserved.
文章类型	Article
资助机构	NSFC(41476126) ; Strategic Priority Research Program of the Chinese Academy of Science(XDA11020405) ; Key Research Program of the Chinese Academy of Sciences(KZZD-EW-14)
收录类别	SCI
语种	英语
关键词[WOS]	MUSSEL MYTILUS-GALLOPROVINCIALIS ; OYSTER CRASSOSTREA-GIGAS ; ANTIMICROBIAL PEPTIDES ; MEDITERRANEAN MUSSEL ; POSITIVE SELECTION ; RAPID EVOLUTION ; INNATE IMMUNITY ; PEARL MUSSEL ; MYTICIN-C ; HEMOCYTES
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000365053300035
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/9539
专题	中国科学院海岸带环境过程与生态修复重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室中国科学院海岸带环境过程与生态修复重点实验室_污染过程与控制实验室海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
通讯作者	Zhao, JM (reprint author), Chinese Acad Sci, Key Lab Coastal Environm Processes & Ecol Remedia, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China. jmzhao@yic.ac.cn
推荐引用方式 GB/T 7714	Wang, Q,Zhang, LB,Yang, DL,et al. Molecular diversity and evolution of defensins in the manila clam Ruditapes philippinarum[J]. FISH & SHELLFISH IMMUNOLOGY,2015,47(1):302-312.
APA	Wang, Q.,Zhang, LB.,Yang, DL.,Yu, Q.,Li, F.,...&Zhao, JM .(2015).Molecular diversity and evolution of defensins in the manila clam Ruditapes philippinarum.FISH & SHELLFISH IMMUNOLOGY,47(1),302-312.
MLA	Wang, Q,et al."Molecular diversity and evolution of defensins in the manila clam Ruditapes philippinarum".FISH & SHELLFISH IMMUNOLOGY 47.1(2015):302-312.

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