Molecular imprinting ratiometric fluorescence sensor for highly selective and sensitive detection of phycocyanin
Wang, XY; Yu, JL; Kang, Q; Shen, DZ; Li, JH; Chen, LX; Shen, DZ (reprint author), Shandong Normal Univ, Collaborat Innovat Ctr Functionalized Probes Chem, Coll Chem Chem Engn & Mat Sci, Key Lab Mol & Nano Probes,Minist Educ,Shandong Pr, Jinan 250014, Peoples R China. dzshen@sdnu.edu.cn; lxchen@yic.ac.cn
2016-03-15
发表期刊BIOSENSORS & BIOELECTRONICS
ISSN0956-5663
卷号77页码:624-630
产权排序[Wang, Xiaoyan; Yu, Jialuo; Kang, Qi; Shen, Dazhong] Shandong Normal Univ, Collaborat Innovat Ctr Functionalized Probes Chem, Coll Chem Chem Engn & Mat Sci, Key Lab Mol & Nano Probes,Minist Educ,Shandong Pr, Jinan 250014, Peoples R China; [Wang, Xiaoyan; Yu, Jialuo; Li, Jinhua; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Wang, Xiaoyan] Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
摘要A facile strategy was developed to prepare molecular imprinting ratiometric fluorescence sensor for highly selective and sensitive detection of phycocyanin (PC) based on fluorescence resonance energy transfer (FRET), via a sal-gel polymerization process using nitrobenzoxadiazole (NBD) as fluorescent signal source. The ratio of two fluorescence peak emission intensities of NBD and PC was utilized to determine the concentration of PC, which could effectively reduce the background interference and fluctuation of diverse conditions. As a result, this sensor obtained high sensitivity with a low detection limit of 0.14 nM within 6 min, and excellent recognition specificity for PC over its analogues with a high imprinting factor of 9.1. Furthermore, the sensor attained high recoveries in the range of 93.8-110.2% at three spiking levels of PC, with precisions below 4.7% in seawater and lake water samples. The developed sensor strategy demonstrated simplicity, reliability, rapidity, high selectivity and high sensitivity, proving to be a feasible way to develop high efficient fluorescence sensors and thus potentially applicable for ultratrace analysis of complicated matrices. (C) 2015 Elsevier B.V. All rights reserved.
关键词Molecular Imprinting Ratiometric Fluorescence Fluorescence Resonance Energy Transfer Phycocyanin
作者部门中科院海岸带环境过程与生态修复重点实验室
DOI10.1016/j.bios.2015.10.019
项目资助者National Natural Science Foundation of China(21175084 ; Research Fund for the Doctoral Program of Higher Education of China(20113704110003) ; Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry ; 21275091 ; 21275158 ; 21477160)
收录类别SCI
关键词[WOS]QUANTUM DOTS ; IONS ; NANOPARTICLES ; POLYMER ; MONOMER ; HYBRID ; PROBE ; SITE ; TNT
文章类型Article
语种英语
WOS研究方向Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS记录号WOS:000366766900090
引用统计
被引频次:22[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/9314
专题中科院海岸带环境过程与生态修复重点实验室
通讯作者Shen, DZ (reprint author), Shandong Normal Univ, Collaborat Innovat Ctr Functionalized Probes Chem, Coll Chem Chem Engn & Mat Sci, Key Lab Mol & Nano Probes,Minist Educ,Shandong Pr, Jinan 250014, Peoples R China. dzshen@sdnu.edu.cn; lxchen@yic.ac.cn
作者单位1.Shandong Normal Univ, Collaborat Innovat Ctr Functionalized Probes Chem, Coll Chem Chem Engn & Mat Sci, Key Lab Mol & Nano Probes,Minist Educ
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat
3.Binzhou Med Univ, Sch Pharm
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Wang, XY,Yu, JL,Kang, Q,et al. Molecular imprinting ratiometric fluorescence sensor for highly selective and sensitive detection of phycocyanin[J]. BIOSENSORS & BIOELECTRONICS,2016,77:624-630.
APA Wang, XY.,Yu, JL.,Kang, Q.,Shen, DZ.,Li, JH.,...&lxchen@yic.ac.cn.(2016).Molecular imprinting ratiometric fluorescence sensor for highly selective and sensitive detection of phycocyanin.BIOSENSORS & BIOELECTRONICS,77,624-630.
MLA Wang, XY,et al."Molecular imprinting ratiometric fluorescence sensor for highly selective and sensitive detection of phycocyanin".BIOSENSORS & BIOELECTRONICS 77(2016):624-630.
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