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题名:
Molecular imprinting ratiometric fluorescence sensor for highly selective and sensitive detection of phycocyanin
作者: Wang, XY; Yu, JL; Kang, Q; Shen, DZ; Jinhua LI(李金花); Chen, LX
刊名: BIOSENSORS & BIOELECTRONICS
ISSN号: 0956-5663
出版日期: 2016-03-15
卷号: 77, 页码:624-630
关键词: Molecular imprinting ; Ratiometric fluorescence ; Fluorescence resonance energy transfer ; Phycocyanin
DOI: 10.1016/j.bios.2015.10.019
产权排序: [Wang, Xiaoyan; Yu, Jialuo; Kang, Qi; Shen, Dazhong] Shandong Normal Univ, Collaborat Innovat Ctr Functionalized Probes Chem, Coll Chem Chem Engn & Mat Sci, Key Lab Mol & Nano Probes,Minist Educ,Shandong Pr, Jinan 250014, Peoples R China; [Wang, Xiaoyan; Yu, Jialuo; Li, Jinhua; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Wang, Xiaoyan] Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
通讯作者: Shen, DZ (reprint author), Shandong Normal Univ, Collaborat Innovat Ctr Functionalized Probes Chem, Coll Chem Chem Engn & Mat Sci, Key Lab Mol & Nano Probes,Minist Educ,Shandong Pr, Jinan 250014, Peoples R China. dzshen@sdnu.edu.cn ; lxchen@yic.ac.cn
作者部门: 中科院海岸带环境过程与生态修复重点实验室
英文摘要: A facile strategy was developed to prepare molecular imprinting ratiometric fluorescence sensor for highly selective and sensitive detection of phycocyanin (PC) based on fluorescence resonance energy transfer (FRET), via a sal-gel polymerization process using nitrobenzoxadiazole (NBD) as fluorescent signal source. The ratio of two fluorescence peak emission intensities of NBD and PC was utilized to determine the concentration of PC, which could effectively reduce the background interference and fluctuation of diverse conditions. As a result, this sensor obtained high sensitivity with a low detection limit of 0.14 nM within 6 min, and excellent recognition specificity for PC over its analogues with a high imprinting factor of 9.1. Furthermore, the sensor attained high recoveries in the range of 93.8-110.2% at three spiking levels of PC, with precisions below 4.7% in seawater and lake water samples. The developed sensor strategy demonstrated simplicity, reliability, rapidity, high selectivity and high sensitivity, proving to be a feasible way to develop high efficient fluorescence sensors and thus potentially applicable for ultratrace analysis of complicated matrices. (C) 2015 Elsevier B.V. All rights reserved.
研究领域[WOS]: Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
项目资助者: National Natural Science Foundation of China(21175084 ; Research Fund for the Doctoral Program of Higher Education of China(20113704110003) ; Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry ; 21275091 ; 21275158 ; 21477160)
关键词[WOS]: QUANTUM DOTS ; IONS ; NANOPARTICLES ; POLYMER ; MONOMER ; HYBRID ; PROBE ; SITE ; TNT
文章类型: Article
收录类别: SCI
语种: 英语
WOS记录号: WOS:000366766900090
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.yic.ac.cn/handle/133337/9314
Appears in Collections:中科院海岸带环境过程与生态修复重点实验室_期刊论文

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作者单位: 1.Shandong Normal Univ, Collaborat Innovat Ctr Functionalized Probes Chem, Coll Chem Chem Engn & Mat Sci, Key Lab Mol & Nano Probes,Minist Educ
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat
3.Binzhou Med Univ, Sch Pharm

Recommended Citation:
Wang, XY,Yu, JL,Kang, Q,et al. Molecular imprinting ratiometric fluorescence sensor for highly selective and sensitive detection of phycocyanin[J]. BIOSENSORS & BIOELECTRONICS,2016,77:624-630.
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