A Near-Infrared Fluorescent Probe for Detection of Nitroxyl in Living Cells

doi:10.1016/S1872-2040(15)60883-0

	A Near-Infrared Fluorescent Probe for Detection of Nitroxyl in Living Cells
	Liu, P; Han, XY; Yu, FB; Chen, LX; Yu, FB (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Processes & Ecol Remedia, Yantai 264003, Peoples R China. fbyu@yic.ac.cn; lxchen@yic.ac.cn
发表期刊	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
ISSN	0253-3820
	2015-12-01
卷号	43 期号:12 页码:1829-1835
关键词	Fluorescent Probe Nitroxyl Cell Analysis Near-infrared Bioimaging
DOI	10.1016/S1872-2040(15)60883-0
产权排序	[Liu Ping; Han Xiao-Yue; Yu Fa-Biao; Chen Ling-Xin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Processes & Ecol Remedia, Yantai 264003, Peoples R China; [Han Xiao-Yue] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
作者部门	中科院海岸带环境过程与生态修复重点实验室
英文摘要	Nitroxyl (HNO), the one-electron reduced and protonated congener of nitric oxide (NO), has been demonstrated with excellent bio-pharmacological effects in cardiovascular disorder treatment, which is distinctive from that of NO. Despite its high reactivity, the accurate detection of HNO is a challenging issue. To resolve this problem, in this work, a near-infrared (NIR) metal-free fluorescent probe, ER-JN, was designed and synthesized for the detection of intracellular HNO concentration in simulated physiological conditions and living cells. The probe was consisted of two moieties, the BF2-chelated tetraarylazadipyrromethane fluorophore (aza-BODIPY) and the HNO recognition unit, diphenylphosphinobenzoyl group. The probe was purified by silica column chromatography eluting with CH2Cl2 to obtain the green solid product with a yield of 28%. The as-prepared probe exhibited high sensitivity, good selectivity and low cytotoxicity, and was applied to fluorescent bio-imaging of HNO in simulated physiological conditions. When used in detection of HNO, quantum yield of the probe increased from 0.01 to 0.35. The linear range was 0-50 mu M, with the detection limit of 0.03 mu M (S/N = 3). With confocal laser scanning microscope imaging analysis, the probe could be used to detect HNO concentration in living cells. Furthermore, the results of flow cytometry confirmed that the probe could be employed for the qualitative and quantitative detection of intracellular HNO level. In this work, we found that probe ER-JN could not only detect HNO in aqueous solution and in living cells, but also target endoplasmic reticulum. We anticipate that ER-JN will provide experimental bases in studying physiological and pathological functions of HNO in cells, in vitro and in vivo.
文章类型	Article
资助机构	Beijing Natural Science Foundation of China(21405172 ; Program of Youth Innovation Promotion Association, Chinese Academy of Sciences(2015170) ; 21575159 ; 21275158)
收录类别	SCI
语种	英语
关键词[WOS]	IN-VIVO ; NITRIC-OXIDE ; HYDROGEN POLYSULFIDES ; HNO ; NO
研究领域[WOS]	Chemistry
WOS记录号	WOS:000368172300004
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/9305
专题	中国科学院海岸带环境过程与生态修复重点实验室
通讯作者	Yu, FB (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Processes & Ecol Remedia, Yantai 264003, Peoples R China. fbyu@yic.ac.cn; lxchen@yic.ac.cn
推荐引用方式 GB/T 7714	Liu, P,Han, XY,Yu, FB,et al. A Near-Infrared Fluorescent Probe for Detection of Nitroxyl in Living Cells[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2015,43(12):1829-1835.
APA	Liu, P,Han, XY,Yu, FB,Chen, LX,Yu, FB ,&lxchen@yic.ac.cn.(2015).A Near-Infrared Fluorescent Probe for Detection of Nitroxyl in Living Cells.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,43(12),1829-1835.
MLA	Liu, P,et al."A Near-Infrared Fluorescent Probe for Detection of Nitroxyl in Living Cells".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 43.12(2015):1829-1835.

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