Iodine-Mediated Etching of Gold Nanorods for Plasmonic ELISA Based on Colorimetric Detection of Alkaline Phosphatase
Zhang, ZY; Chen, ZP; Wang, SS; Cheng, FB; Chen, LX; Chen, ZP (reprint author), Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Shandong, Peoples R China. zhpchen@yic.ac.cn
2015-12-23
发表期刊ACS APPLIED MATERIALS & INTERFACES
ISSN1944-8244
卷号7期号:50页码:27639-27645
产权排序[Zhang, Zhiyang; Chen, Zhaopeng; Wang, Shasha; Chen, Lingxin] Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Shandong, Peoples R China; [Zhang, Zhiyang; Wang, Shasha] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Cheng, Fangbin] Yantai Univ, Ocean Sch, Yantai 264005, Peoples R China
摘要Here, we propose a plasmonic enzyme-linked immunosorbent assay (ELISA) based on highly sensitive colorimetric detection of alkaline phosphatase (ALP), which is achieved by iodine-mediated etching of gold nanorods (AuNRs). Once the sandwich-type immunocomplex is formed, the ALP bound on the polystyrene microwells will hydrolyze ascorbic acid 2-phosphate into ascorbic acid. Subsequently, iodate is reduced to iodine, a moderate oxidant, which etches AuNRs from rod to sphere in shape. The shape change of AuNRs leads to a blue-shift of longitudinal localized surface plasmon resonance. As a result, the solution of AuNRs changes from blue to red. Benefiting from the highly sensitive detection of ALP, the proposed plasmonic ELISA has achieved an ultralow detection limit (100 pg/mL) for human immunoglobulin G (IgG). Importantly, the visual detection limit (3.0 ng/mL) allows the rapid differential diagnosis with the naked eye. The further detection of human IgG in fetal bovine serum indicates its applicability to the determination of low abundance protein in complex biological samples.
关键词Gold Nanorods Etching Iodine Alkaline Phosphatase Plasmonic Elisa
作者部门中科院海岸带环境过程与生态修复重点实验室
DOI10.1021/acsami.5b07344
项目资助者Department of Science and Technology of Shandong Province(BS2009DX006) ; NSFC(21575159 ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA11020702) ; 21275158)
收录类别SCI
关键词[WOS]VISUAL DETECTION ; ULTRASENSITIVE DETECTION ; SILVER ENHANCEMENT ; NANOPARTICLES ; IMMUNOASSAY ; ASSAY ; AGGREGATION ; GLUCOSE ; AMPLIFICATION ; SPECTROSCOPY
文章类型Article
语种英语
WOS研究方向Science & Technology - Other Topics ; Materials Science
WOS记录号WOS:000367561500010
引用统计
被引频次:33[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/9299
专题中科院海岸带环境过程与生态修复重点实验室
通讯作者Chen, ZP (reprint author), Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Shandong, Peoples R China. zhpchen@yic.ac.cn
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GB/T 7714
Zhang, ZY,Chen, ZP,Wang, SS,et al. Iodine-Mediated Etching of Gold Nanorods for Plasmonic ELISA Based on Colorimetric Detection of Alkaline Phosphatase[J]. ACS APPLIED MATERIALS & INTERFACES,2015,7(50):27639-27645.
APA Zhang, ZY,Chen, ZP,Wang, SS,Cheng, FB,Chen, LX,&Chen, ZP .(2015).Iodine-Mediated Etching of Gold Nanorods for Plasmonic ELISA Based on Colorimetric Detection of Alkaline Phosphatase.ACS APPLIED MATERIALS & INTERFACES,7(50),27639-27645.
MLA Zhang, ZY,et al."Iodine-Mediated Etching of Gold Nanorods for Plasmonic ELISA Based on Colorimetric Detection of Alkaline Phosphatase".ACS APPLIED MATERIALS & INTERFACES 7.50(2015):27639-27645.
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