| Iodine-Mediated Etching of Gold Nanorods for Plasmonic ELISA Based on Colorimetric Detection of Alkaline Phosphatase | |
Zhang, ZY1,2 ; Chen, ZP1; Wang, SS1,2; Cheng, FB3; Chen, LX1
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| 发表期刊 | ACS APPLIED MATERIALS & INTERFACES
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| ISSN | 1944-8244 |
| 2015-12-23 | |
| 卷号 | 7期号:50页码:27639-27645 |
| 关键词 | Gold Nanorods Etching Iodine Alkaline Phosphatase Plasmonic Elisa |
| DOI | 10.1021/acsami.5b07344 |
| 产权排序 | [Zhang, Zhiyang; Chen, Zhaopeng; Wang, Shasha; Chen, Lingxin] Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Shandong, Peoples R China; [Zhang, Zhiyang; Wang, Shasha] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Cheng, Fangbin] Yantai Univ, Ocean Sch, Yantai 264005, Peoples R China |
| 作者部门 | 中科院海岸带环境过程与生态修复重点实验室 |
| 英文摘要 | Here, we propose a plasmonic enzyme-linked immunosorbent assay (ELISA) based on highly sensitive colorimetric detection of alkaline phosphatase (ALP), which is achieved by iodine-mediated etching of gold nanorods (AuNRs). Once the sandwich-type immunocomplex is formed, the ALP bound on the polystyrene microwells will hydrolyze ascorbic acid 2-phosphate into ascorbic acid. Subsequently, iodate is reduced to iodine, a moderate oxidant, which etches AuNRs from rod to sphere in shape. The shape change of AuNRs leads to a blue-shift of longitudinal localized surface plasmon resonance. As a result, the solution of AuNRs changes from blue to red. Benefiting from the highly sensitive detection of ALP, the proposed plasmonic ELISA has achieved an ultralow detection limit (100 pg/mL) for human immunoglobulin G (IgG). Importantly, the visual detection limit (3.0 ng/mL) allows the rapid differential diagnosis with the naked eye. The further detection of human IgG in fetal bovine serum indicates its applicability to the determination of low abundance protein in complex biological samples. |
| 文章类型 | Article |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | VISUAL DETECTION ; ULTRASENSITIVE DETECTION ; SILVER ENHANCEMENT ; NANOPARTICLES ; IMMUNOASSAY ; ASSAY ; AGGREGATION ; GLUCOSE ; AMPLIFICATION ; SPECTROSCOPY |
| 研究领域[WOS] | Science & Technology - Other Topics ; Materials Science |
| WOS记录号 | WOS:000367561500010 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/9299 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心 中国科学院海岸带环境过程与生态修复重点实验室 |
| 通讯作者 | Chen, ZP |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Yantai Univ, Ocean Sch, Yantai 264005, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, ZY,Chen, ZP,Wang, SS,et al. Iodine-Mediated Etching of Gold Nanorods for Plasmonic ELISA Based on Colorimetric Detection of Alkaline Phosphatase[J]. ACS APPLIED MATERIALS & INTERFACES,2015,7(50):27639-27645. |
| APA | Zhang, ZY,Chen, ZP,Wang, SS,Cheng, FB,&Chen, LX.(2015).Iodine-Mediated Etching of Gold Nanorods for Plasmonic ELISA Based on Colorimetric Detection of Alkaline Phosphatase.ACS APPLIED MATERIALS & INTERFACES,7(50),27639-27645. |
| MLA | Zhang, ZY,et al."Iodine-Mediated Etching of Gold Nanorods for Plasmonic ELISA Based on Colorimetric Detection of Alkaline Phosphatase".ACS APPLIED MATERIALS & INTERFACES 7.50(2015):27639-27645. |
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| Iodine-Mediated Etch（2800KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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