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题名:
Iodine-Mediated Etching of Gold Nanorods for Plasmonic ELISA Based on Colorimetric Detection of Alkaline Phosphatase
作者: Zhang, ZY; Chen, ZhaoPeng(陈兆鹏); Wang, SS; Cheng, FB; Chen, LX
刊名: ACS APPLIED MATERIALS & INTERFACES
ISSN号: 1944-8244
出版日期: 2015-12-23
卷号: 7, 期号:50, 页码:27639-27645
关键词: gold nanorods ; etching ; iodine ; alkaline phosphatase ; plasmonic ELISA
DOI: 10.1021/acsami.5b07344
产权排序: [Zhang, Zhiyang; Chen, Zhaopeng; Wang, Shasha; Chen, Lingxin] Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Shandong, Peoples R China; [Zhang, Zhiyang; Wang, Shasha] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Cheng, Fangbin] Yantai Univ, Ocean Sch, Yantai 264005, Peoples R China
通讯作者: Chen, ZP (reprint author), Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Shandong, Peoples R China. zhpchen@yic.ac.cn
作者部门: 中科院海岸带环境过程与生态修复重点实验室
英文摘要: Here, we propose a plasmonic enzyme-linked immunosorbent assay (ELISA) based on highly sensitive colorimetric detection of alkaline phosphatase (ALP), which is achieved by iodine-mediated etching of gold nanorods (AuNRs). Once the sandwich-type immunocomplex is formed, the ALP bound on the polystyrene microwells will hydrolyze ascorbic acid 2-phosphate into ascorbic acid. Subsequently, iodate is reduced to iodine, a moderate oxidant, which etches AuNRs from rod to sphere in shape. The shape change of AuNRs leads to a blue-shift of longitudinal localized surface plasmon resonance. As a result, the solution of AuNRs changes from blue to red. Benefiting from the highly sensitive detection of ALP, the proposed plasmonic ELISA has achieved an ultralow detection limit (100 pg/mL) for human immunoglobulin G (IgG). Importantly, the visual detection limit (3.0 ng/mL) allows the rapid differential diagnosis with the naked eye. The further detection of human IgG in fetal bovine serum indicates its applicability to the determination of low abundance protein in complex biological samples.
研究领域[WOS]: Science & Technology - Other Topics ; Materials Science
项目资助者: Department of Science and Technology of Shandong Province(BS2009DX006) ; NSFC(21575159 ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA11020702) ; 21275158)
关键词[WOS]: VISUAL DETECTION ; ULTRASENSITIVE DETECTION ; SILVER ENHANCEMENT ; NANOPARTICLES ; IMMUNOASSAY ; ASSAY ; AGGREGATION ; GLUCOSE ; AMPLIFICATION ; SPECTROSCOPY
文章类型: Article
收录类别: SCI
语种: 英语
WOS记录号: WOS:000367561500010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.yic.ac.cn/handle/133337/9299
Appears in Collections:中科院海岸带环境过程与生态修复重点实验室_期刊论文

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Recommended Citation:
Zhang, ZY,Chen, ZP,Wang, SS,et al. Iodine-Mediated Etching of Gold Nanorods for Plasmonic ELISA Based on Colorimetric Detection of Alkaline Phosphatase[J]. ACS APPLIED MATERIALS & INTERFACES,2015,7(50):27639-27645.
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