Naked-eye sensitive ELISA-like assay based on gold-enhanced peroxidase-like immunogold activity

doi:10.1007/s00216-015-9219-8

	Naked-eye sensitive ELISA-like assay based on gold-enhanced peroxidase-like immunogold activity
	Wang, SS ; Chen, ZP; Choo, J ; Chen, LX; Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China. lxchen@yic.ac.cn
发表期刊	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
ISSN	1618-2642
	2016-02-01
卷号	408 期号:4 页码:1015-1022
关键词	Gold Nanoparticles Peroxidase-like Activity Colorimetric Immunoassay Human Igg Gold Enhancement
DOI	10.1007/s00216-015-9219-8
产权排序	[Wang, Shasha; Chen, Zhaopeng; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Choo, Jaebum] Hanyang Univ, Dept Bionano Technol, Ansan 426791, South Korea; [Wang, Shasha] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
作者部门	中科院海岸带环境过程与生态修复重点实验室
英文摘要	A naked-eye sensitive ELISA-like assay was developed based on gold-enhanced peroxidase-like activity of gold nanoparticles (AuNPs). Using human IgG (H-IgG) as an analytical model, goat anti-human IgG antibody (anti-IgG) adsorbed on microtiter plate and AuNPs-labeled anti-IgG acted as capture antibody and detection antibody, respectively. Because the surfaces of AuNPs were blocked by protein molecules, the peroxidase-like activity of AuNPs was almost inhibited, evaluated by the catalytic oxidation of peroxidase enzyme substrate 3,3',5,5'-tetramethylbenzidine (TMB), which could produce a bright blue color in the presence of H2O2. Fortunately, the catalytic ability of AuNPs was dramatically increased by the deposition of gold due to the formation of a new gold shell on immunogold. Under optimal reaction conditions, the colorimetric immunoassay presented a good linear relationship in the range of 0.7-100 ng/mL and the limit of detection (LOD) of 0.3 ng/mL calculated by 3 sigma/S for UV-vis detection, and obtained LOD of 5 ng/mL for naked-eye detection. The obtained results were competitive with conventional sandwich ELISA with the LOD of 1.6 ng/mL. Furthermore, this developed colorimetric immunoassay was successfully applied to diluted human serum and fetal bovine serum samples, and predicted a broad prospect for the use of peroxidase-like activity involving nanomaterials in bioassay and diagnostics.
文章类型	Article
资助机构	National Natural Science Foundation of China(21275158 ; National Research Foundation of Korea(2008-0061891 ; 100 Talents Program of the Chinese Academy of Sciences ; 21575159) ; 2009-00426)
收录类别	SCI
语种	英语
关键词[WOS]	LINKED-IMMUNOSORBENT-ASSAY ; COLORIMETRIC IMMUNOASSAY ; HORSERADISH-PEROXIDASE ; IMMUNOGLOBULIN-G ; NANOPARTICLES ; IGG ; REDUCTION ; GRAPHENE ; LABELS
研究领域[WOS]	Biochemistry & Molecular Biology ; Chemistry
WOS记录号	WOS:000368720600001
引用统计	被引频次：56[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/9292
专题	中国科学院海岸带环境过程与生态修复重点实验室
通讯作者	Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China. lxchen@yic.ac.cn
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat 2.Hanyang Univ, Dept Bionano Technol 3.Univ Chinese Acad Sci
推荐引用方式 GB/T 7714	Wang, SS,Chen, ZP,Choo, J,et al. Naked-eye sensitive ELISA-like assay based on gold-enhanced peroxidase-like immunogold activity[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2016,408(4):1015-1022.
APA	Wang, SS,Chen, ZP,Choo, J,Chen, LX,&Chen, LX .(2016).Naked-eye sensitive ELISA-like assay based on gold-enhanced peroxidase-like immunogold activity.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,408(4),1015-1022.
MLA	Wang, SS,et al."Naked-eye sensitive ELISA-like assay based on gold-enhanced peroxidase-like immunogold activity".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 408.4(2016):1015-1022.

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