Critical roles of sea cucumber C-type lectin in non-self recognition and bacterial clearance

doi:10.1016/j.fsi.2015.05.037

	Critical roles of sea cucumber C-type lectin in non-self recognition and bacterial clearance
	Wei, XM; Liu, XQ; Yang, JM; Wang, S; Sun, GH; Yang, JL; Yang, JL (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China. jlyang@yic.ac.cn
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
	2015-08-01
卷号	45 期号:2 页码:791-799
关键词	Apostichopus Japonicus C-type Lectin Immune Recognition Pattern Recognition Receptor Bacterial Clearance
DOI	10.1016/j.fsi.2015.05.037
产权排序	[Wei, Xiumei; Liu, Xiangquan; Yang, Jianmin; Wang, Sheng; Sun, Guohua] Shandong Marine Resource & Environm Res Inst, Shandong Prov Key Lab Marine Ecol Restorat, Yantai 264006, Peoples R China; [Yang, Jialong] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
作者部门	中科院烟台海岸带研究所知识产出
英文摘要	C-type lectin is one important pattern recognition receptor (PRR) that plays crucial roles in multiple immune responses. A C-type lectin from sea cucumber Apostichopus japonicus (AjCTL-1) was characterized in the present study. The amino acid sequence of AjCTL-1 shared high similarities with other C-type lectins from invertebrates and vertebrates. The C-type lectin domain (CTLD) of AjCTL-1 contained a Ca2+-binding site 2 and four conserved cysteine residues. AjCTL-1 mRNA expression patterns in tissues and after bacterial challenge were then analysed. Quantitative PCR revealed that AjCTL-1 mRNA was widely expressed in the tested tissues of healthy sea cucumber. The highest expression level occurred in gonad followed by body wall, coelomocytes, tentacle, intestinum and longitudinal muscle, and the lowest expression level was in respiratory tree. AjCTL-1 mRNA expression in coelomocytes was significantly induced by gram-negative Listonella anguillarum and gram-positive Micrococcus luteus, with different up-regulation patterns post-challenge. Recombinant AjCTL-1 exhibited the ability to bind peptidoglycan directly, agglutinate M. luteus, Staphylococcus aureus and Escherichia coli, in a Ca2+-dependant manner, and enhance the phagocytosis of coelomocytes against E. coli in vitro. The results indicated that AjCTL-1 could act as a PRR in Apostichopus japonicus and had critical roles in non-self recognition and bacterial clearance against invading microbes. (C) 2015 Elsevier Ltd. All rights reserved.
文章类型	Article
收录类别	SCI
语种	英语
关键词[WOS]	SCALLOP ARGOPECTEN-IRRADIANS ; SHRIMP LITOPENAEUS-VANNAMEI ; MANNAN-BINDING LECTIN ; MOLLUSK SOLEN-GRANDIS ; INNATE IMMUNE-SYSTEM ; AMINO-ACID-SEQUENCE ; APOSTICHOPUS-JAPONICUS ; EXPRESSION ANALYSIS ; MOLECULAR-CLONING ; CHLAMYS-FARRERI
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000358626800067
引用统计	被引频次：26[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/9279
专题	中国科学院烟台海岸带研究所知识产出
通讯作者	Yang, JL (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China. jlyang@yic.ac.cn
推荐引用方式 GB/T 7714	Wei, XM,Liu, XQ,Yang, JM,et al. Critical roles of sea cucumber C-type lectin in non-self recognition and bacterial clearance[J]. FISH & SHELLFISH IMMUNOLOGY,2015,45(2):791-799.
APA	Wei, XM.,Liu, XQ.,Yang, JM.,Wang, S.,Sun, GH.,...&Yang, JL .(2015).Critical roles of sea cucumber C-type lectin in non-self recognition and bacterial clearance.FISH & SHELLFISH IMMUNOLOGY,45(2),791-799.
MLA	Wei, XM,et al."Critical roles of sea cucumber C-type lectin in non-self recognition and bacterial clearance".FISH & SHELLFISH IMMUNOLOGY 45.2(2015):791-799.

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